Anti-MTCO2抗体(ab101802)

概述

  • 产品名称Anti-MTCO2抗体
    参阅全部 MTCO2 一抗
  • 描述
    兔多克隆抗体to MTCO2
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Cytochrome C oxidase subunit II.

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Liver (Human) Tissue; Heart (Human) Tissue; Skeletal Muscle (Human) Tissue; Brain (Human) Tissue; Testis (Human) Tissue; MCF7 Whole Cell; HeLa Whole Cell.

性能

应用

Our Abpromise guarantee covers the use of ab101802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 25 kDa).

靶标

  • 功能Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
  • 疾病相关Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
  • 序列相似性Belongs to the cytochrome c oxidase subunit 2 family.
  • 细胞定位Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COII antibody
    • COX2 antibody
    • COX2_HUMAN antibody
    • COXII antibody
    • Cytochrome c oxidase II antibody
    • Cytochrome c oxidase polypeptide II antibody
    • Cytochrome c oxidase subunit 2 antibody
    • MT CO2 antibody
    • MT-CO2 antibody
    • MTCO2 antibody
    see all

Anti-MTCO2 antibody 图像

  • All lanes : Anti-MTCO2 antibody (ab101802) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 4 : Human brain tissue lysate - total protein (ab29466)
    Lane 5 : Human testis tissue lysate - total protein (ab30257)
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,37 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab101802 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101802, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 1µg/ml.

Anti-MTCO2 antibody (ab101802)参考文献

ab101802 has not yet been referenced specifically in any publications.

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The immunogens are as follows;

ab79393; A synthetic peptide corresponding to residues in human Cytochrome C oxidase subunit II
ab109739; A synthetic peptide corresponding to residues in Human Cytochro...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (Cre4 vs Cre4 rho-0)
Loading amount 26 µg
Specification Cre4 vs Cre4 rho-0
Gel Running Conditions Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Aug 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HeLa WT vs HeLa rho-0)
Loading amount 13 µg
Specification HeLa WT vs HeLa rho-0
Gel Running Conditions Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Aug 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

提交于 Jun 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"