Full length native protein (purified) corresponding to Human MTCO2. Full length native Human Cytochrome C oxidase subunit II purified by SDS PAGE.
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Our Abpromise guarantee covers the use of ab110258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
IHC-Fr | Use at an assay dependent concentration. | |
WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. | |
ICC/IF | Use a concentration of 5 - 10 µg/ml. (heat-induced antigen-retrieval improves signal) | |
Flow Cyt | Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ab110258 staining human lung sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab110258 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence analysis of human embryonic lung derived fibroblasts (MRC5) labelling Cytochrome C oxidase subunit II with ab110258 at 5 µg/ml. An Alexa Fluor® 488-conjugated goat anti-mouse IgG2a isotype specific secondary antibody was used at 2 µg/ml.
An isotype control antibody, at 1 µg/ml, staining in HeLa cells (Red).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"