- 数据表
- 具体的参考文献 (80)
- 实验方案
概述
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产品名称Anti-MTCO2抗体[12C4F12]
参阅全部 MTCO2 一抗 -
描述小鼠单克隆抗体[12C4F12] to MTCO2
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经测试应用适用于: IHC-P, IHC-Fr, WB, ICC/IF, Flow Cytmore details
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种属反应性与反应: Human
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免疫原
Full length native protein (purified) corresponding to Human MTCO2. Full length native Human Cytochrome C oxidase subunit II purified by SDS PAGE.
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阳性对照
- WB: Isolated mitochondria from human heart. ICC/IF: Cultured human embryonic lung derived fibroblasts. Flow Cyt: HeLa cells.
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常规说明
Product was previously marketed under the MitoSciences sub-brand.
Alternative versions available:
Anti-MTCO2 antibody (Alexa Fluor® 488) [12C4F12] (ab197958)
Anti-MTCO2 antibody (HRP) [12C4F12] (ab197959)
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C.
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存储溶液Preservative: 0.02% Sodium azide
Constituent: HBS -
Concentration information loading... -
纯度IgG fraction
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纯化说明ab110258 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110258 was judged near homogeneity by SDS-PAGE.
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克隆单克隆
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克隆编号12C4F12
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同种型IgG2a
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轻链类型kappa
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研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab110258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. | |
| ICC/IF | Use a concentration of 5 - 10 µg/ml. (heat-induced antigen-retrieval improves signal) | |
| Flow Cyt | Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
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疾病相关Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
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序列相似性Belongs to the cytochrome c oxidase subunit 2 family.
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细胞定位Mitochondrion inner membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 4513 Human
- Omim: 516040 Human
- SwissProt: P00403 Human
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别名
- COII antibody
- COX2 antibody
- COX2_HUMAN antibody
see all
Anti-MTCO2 antibody [12C4F12] 图像
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![Western blot - Anti-MTCO2 antibody [12C4F12] (ab110258)](http://www.abcam.cn/ps/products/110/ab110258/Images/Cytochrome-C-oxidase-subunit-II-Primary-antibodies-ab110258-4.jpg)
Western blot - Anti-MTCO2 antibody [12C4F12] (ab110258)Anti-MTCO2 antibody [12C4F12] (ab110258) at 1 µg/ml + Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg
Predicted band size : 26 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [12C4F12] (ab110258)](http://www.abcam.cn/ps/products/110/ab110258/Images/Cytochrome-C-oxidase-subunit-II-Primary-antibodies-ab110258-16.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [12C4F12] (ab110258)This image is courtesy of an Abreview submitted by Carl Hobbsab110258 staining human lung sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab110258 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [12C4F12] (ab110258)](http://www.abcam.cn/ps/products/110/ab110258/Images/Cytochrome-C-oxidase-subunit-II-Primary-antibodies-ab110258-5.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [12C4F12] (ab110258)Immunocytochemistry/Immunofluorescence analysis of human embryonic lung derived fibroblasts (MRC5) labelling Cytochrome C oxidase subunit II with ab110258 at 5 µg/ml. An Alexa Fluor® 488-conjugated goat anti-mouse IgG2a isotype specific secondary antibody was used at 2 µg/ml.
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![Flow Cytometry - Anti-MTCO2 antibody [12C4F12] (ab110258)](http://www.abcam.cn/ps/products/110/ab110258/Images/Cytochrome-C-oxidase-subunit-II-Primary-antibodies-ab110258-6.jpg)
Flow Cytometry - Anti-MTCO2 antibody [12C4F12] (ab110258)ab110258, at 1 µg/ml, staining Cytochrome C oxidase subunit II in HeLa cells by Flow Cytometry (Blue).An isotype control antibody, at 1 µg/ml, staining in HeLa cells (Red).
实验方案
Anti-MTCO2 antibody [12C4F12] (ab110258)参考文献
This product has been referenced in:
- Gómez-Serrano M et al. Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetes. Redox Biol 11:415-428 (2017). WB ; Human . Read more (PubMed: 28064117) »
- Jones E et al. A threshold of transmembrane potential is required for mitochondrial dynamic balance mediated by DRP1 and OMA1. Cell Mol Life Sci 74:1347-1363 (2017). WB ; Human . Read more (PubMed: 27858084) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"