Anti-MTCO2抗体[12C4F12] (ab110258)

Abreviews (13)Q&A (6)Specific References (96)

概述

  • 产品名称
    Anti-MTCO2抗体[12C4F12]
    参阅全部 MTCO2 一抗
  • 描述
    小鼠单克隆抗体[12C4F12] to MTCO2
  • 宿主
    Mouse
  • 经测试应用
    适用于: IHC-P, IHC-Fr, WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human MTCO2. Full length native Human Cytochrome C oxidase subunit II purified by SDS PAGE.

  • 阳性对照
    • WB: Isolated mitochondria from human heart. ICC/IF: Cultured human embryonic lung derived fibroblasts. Flow Cyt: HeLa cells.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

应用

Our Abpromise guarantee covers the use of ab110258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
ICC/IF Use a concentration of 5 - 10 µg/ml. (heat-induced antigen-retrieval improves signal)
Flow Cyt Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
  • 疾病相关
    Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
  • 序列相似性
    Belongs to the cytochrome c oxidase subunit 2 family.
  • 细胞定位
    Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • COII antibody
      • COX2 antibody
      • COX2_HUMAN antibody
      • COXII antibody
      • Cytochrome c oxidase II antibody
      • Cytochrome c oxidase polypeptide II antibody
      • Cytochrome c oxidase subunit 2 antibody
      • MT CO2 antibody
      • MT-CO2 antibody
      • MTCO2 antibody
      see all

    图片

    • Western blot - Anti-MTCO2 antibody [12C4F12] (ab110258)
      Western blot - Anti-MTCO2 antibody [12C4F12] (ab110258)
      Anti-MTCO2 antibody [12C4F12] (ab110258) at 1 µg/ml + Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg

      Predicted band size: 26 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [12C4F12] (ab110258)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO2 antibody [12C4F12] (ab110258)This image is courtesy of an Abreview submitted by Carl Hobbs

      ab110258 staining human lung sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab110258 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [12C4F12] (ab110258)
      Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [12C4F12] (ab110258)

      Immunocytochemistry/Immunofluorescence analysis of human embryonic lung derived fibroblasts (MRC5) labelling Cytochrome C oxidase subunit II with ab110258 at 5 µg/ml. An Alexa Fluor® 488-conjugated goat anti-mouse IgG2a isotype specific secondary antibody was used at 2 µg/ml.

    • Flow Cytometry - Anti-MTCO2 antibody [12C4F12] (ab110258)
      Flow Cytometry - Anti-MTCO2 antibody [12C4F12] (ab110258)
      ab110258, at 1 µg/ml, staining Cytochrome C oxidase subunit II in HeLa cells by Flow Cytometry (Blue).

      An isotype control antibody, at 1 µg/ml, staining in HeLa cells (Red).

    文献

    This product has been referenced in:

    See all 96 Publications for this product

    发表研究结果有使用 ab110258？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    19 reviews or Q&A

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    Votes: Highest First

    Western blot abreview for Anti-MTCO2 antibody [12C4F12]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Placenta)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    Placenta
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
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    Jeremiah Gaudet

    Verified customer

    提交于 Mar 23 2018

    Western blot abreview for Anti-MTCO2 antibody [12C4F12]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Placenta)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    Placenta
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
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    Jeremiah Gaudet

    Verified customer

    提交于 Mar 22 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MTCO2 antibody [12C4F12]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS Cells)
    Permeabilization
    Yes - 0.2% NP-40
    Specification
    U2OS Cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
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    Dr. Michael Munson

    Verified customer

    提交于 Sep 21 2017

    Western blot abreview for Anti-MTCO2 antibody [12C4F12]

     Excellent
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - other (U2OS Cells - Cytosol v Membrane Fractionation)
    Gel Running Conditions
    Reduced Denaturing (4-20% Acrylamide)
    Loading amount
    30 µg
    Specification
    U2OS Cells - Cytosol v Membrane Fractionation
    Blocking step
    Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More
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    Dr. Michael Munson

    Verified customer

    提交于 Sep 08 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MTCO2 antibody [12C4F12]

     Inconclusive
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 4°C
    Sample
    Zebrafish Cell (Whole Body)
    Specification
    Whole Body
    Permeabilization
    Yes - Triton X-100
    Fixative
    Paraformaldehyde
    Read More
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    Abcam user community

    Verified customer

    提交于 Nov 25 2014

    Western blot abreview for Anti-MTCO2 antibody [12C4F12]

     Inconclusive
    Abreviews
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Rat Cell lysate - whole cell (Primary Neurons)
    Specification
    Primary Neurons
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
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    Abcam user community

    Verified customer

    提交于 Aug 22 2014

    Western blot abreview for Anti-MTCO2 antibody [12C4F12]

     Inconclusive
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Zebrafish Tissue lysate - whole (Larvae 5hpf)
    Specification
    Larvae 5hpf
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
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    Abcam user community

    Verified customer

    提交于 Aug 22 2014

    Western blot abreview for Anti-MTCO2 antibody [12C4F12 ]

     Inconclusive
    Abreviews
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (15% GEL)
    Sample
    Human Cell lysate - whole cell (H460)
    Specification
    H460
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Read More
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    Abcam user community

    Verified customer

    提交于 May 26 2014

    Western blot abreview for Anti-MTCO2 antibody [12C4F12 ]

     Inconclusive
    Abreviews
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (15% GEL)
    Sample
    Mouse Cell lysate - whole cell (mouse Embryonic Fibroblasts)
    Specification
    mouse Embryonic Fibroblasts
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Read More
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    Abcam user community

    Verified customer

    提交于 May 22 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-MTCO2 antibody [12C4F12 ]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Cardiac Muscle)
    Specification
    Cardiac Muscle
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Read More
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    Abcam user community

    Verified customer

    提交于 Apr 08 2013

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