概述

  • 产品名称Anti-MTCO1抗体
    参阅全部 MTCO1 一抗
  • 描述
    兔多克隆抗体to MTCO1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A synthetic peptide corresponding to a sequence at the N-terminal of Human MTCO1.

性能

应用

Our Abpromise guarantee covers the use of ab90668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa.

靶标

  • 功能Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.
  • 通路Energy metabolism; oxidative phosphorylation.
  • 疾病相关Defects in MT-CO1 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
    Defects in MT-CO1 are a cause of anemia sideroblastic acquired idiopathic (AISA) [MIM:516030]; a disease characterized by inadequate formation of heme and excessive accumulation of iron in mitochondria.
    Defects in MT-CO1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
    Defects in MT-CO1 are associated with recurrent myoglobinuria mitochondrial (RM-MT) [MIM:550500]. Recurrent myoglobinuria is characterized by recurrent attacks of rhabdomyolysis (necrosis or disintegration of skeletal muscle) associated with muscle pain and weakness, and followed by excretion of myoglobin in the urine.
    Defects in MT-CO1 are a cause of deafness sensorineural mitochondrial (DFNM) [MIM:500008]. DFNM is a form of non-syndromic deafness with maternal inheritance. Affected individuals manifest progressive, postlingual, sensorineural hearing loss involving high frequencies.
    Defects in MT-CO1 are a cause of colorectal cancer (CRC) [MIM:114500].
  • 序列相似性Belongs to the heme-copper respiratory oxidase family.
  • 细胞定位Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COI antibody
    • COX I antibody
    • COX1 antibody
    • COX1_HUMAN antibody
    • COXI antibody
    • Cytochrome c oxidase polypeptide I antibody
    • Cytochrome c oxidase subunit 1 antibody
    • Cytochrome C Oxidase subunit I antibody
    • Mitochondrially encoded cytochrome c oxidase I antibody
    • MT CO1 antibody
    • MT-CO1 antibody
    • MTCO 1 antibody
    • MTCO1 antibody
    see all

Anti-MTCO1 antibody 图像

  • All lanes : Anti-MTCO1 antibody (ab90668) at 1 µg/ml

    Lane 1 : Whole cell lysates prepared from MCF-7 cells.
    Lane 2 : Whole cell lysates prepared from Hela cells.
    Lane 3 : Whole cell lysates prepared from Jurkat cells.
    Lane 4 : Whole cell lysates prepared from SMMC cells.
    Lane 5 : Whole cell lysates prepared from Colo320 cells.

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution

    Predicted band size : 57 kDa

Anti-MTCO1 antibody (ab90668)参考文献

This product has been referenced in:
  • Zhang X  et al. Induction of mitochondrial dysfunction as a strategy for targeting tumour cells in metabolically compromised microenvironments. Nat Commun 5:3295 (2014). WB . Read more (PubMed: 24548894) »
  • Rice MW  et al. Assessment of cytochrome C oxidase dysfunction in the substantia nigra/ventral tegmental area in schizophrenia. PLoS One 9:e100054 (2014). WB ; Human . Read more (PubMed: 24941246) »

See all 2 Publications for this product

Product Wall

I am sorry for the delay in getting back to you.

I have now checked the other antibodies we have against this target and unfortunately none would be predicted to cross react with the Drosophila protein except the rabbit polyclonal ab90668. Ho...

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Thank you for contacting us and your interest in our products.

We have not tested the Anti-MTCO1 antibody [1D6E1A8] (ab14705) for its cross reactivity with Drosophila melanogaster. The antibody has been raised against the full length human MT...

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Application Western blot
Sample Human Cell lysate - other (Mitochondrial fractions of HeLa WT and HeLa rho-0)
Loading amount 26 µg
Specification Mitochondrial fractions of HeLa WT and HeLa rho-0
Gel Running Conditions Reduced Denaturing (Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Verified customer

提交于 Aug 17 2012

Vielen Dank für Ihre Antwort.

Es freut mich dass ich Ihnen weiterhelfen konnte.

Meine Vorhersage ist, dass Ihr Blot gutklappen wird, wenn das Kochen weggelassen wird.

Ich werde Ihr Feedback bezüglich der Datenbl&...

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Vielen Dank für Ihre Anfrage und die Zusendung Ihres Blots.

Ich freue mich Ihnen bestätigen zu können, dass MTCO1 immer unter der errechneten Größe von 57kDa läuft.

MTCO1 ist ein extrem hydrophobes Pro...

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Thank you for contacting us. These antibodies are indeed raised in mouse and these antibodies will work better in human, mouse, rat cells and tissue sections. In case you are planning to use these antibodies on mouse tissue sections then we wou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"