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Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human MTCO1.
(Peptide available as ab45933.)
Our Abpromise guarantee covers the use of ab45918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 57 kDa).
There is evidence in the scientific literature to support the observation of MTCO1 protein at approx. 35-kDa on Western Blot (see PMID: 21437181).
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab45918 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Ab45918 detects a band at 35-kDa. While this differs to its predicted molecular weight of 57-kDa, the band observed is consistent with what has been described in the literature (PMID: 21437181).