概述

  • 产品名称
  • 描述
    兔多克隆抗体to MTA3
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 400-450 (

    CRLCAICWLYWKKYGGLKMPTQSEEEKLSPSPTTEDPRVRSHVSRQAMQG M

    ) of Human MTA3 (Swiss-Prot entry Q9BTC8, GeneID 57504)

  • 阳性对照
    • Human controls: Breast Carcinoma, Colon Carcinoma, Prostate Carcinoma, Testicular Carcinoma
  • 常规说明

    Concentration is optimized for IHC and not determined

性能

应用

Our Abpromise guarantee covers the use of ab87275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Use under non reducing condition. Predicted molecular weight: 67 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能
    Plays a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.
  • 组织特异性
    No expression in nonepithelial cells.
  • 序列相似性
    Contains 1 BAH domain.
    Contains 1 ELM2 domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 SANT domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110002J22Rik antibody
    • fj99h01 antibody
    • KIAA1266 antibody
    • Metastasis associated 1 family, member 3 antibody
    • Metastasis associated 3 antibody
    • Metastasis associated family, member 3 antibody
    • Metastasis associated gene 3 antibody
    • Metastasis associated gene family, member 3 antibody
    • Metastasis associated protein MTA3 antibody
    • Metastasis-associated protein MTA3 antibody
    • MGC56396 antibody
    • MGC77410 antibody
    • mKIAA1266 antibody
    • mta1 antibody
    • Mta3 antibody
    • MTA3 metastasis associated 1 family, member 3 antibody
    • Mta3 metastasis associated 3 antibody
    • MTA3_HUMAN antibody
    • wu:fj99h01 antibody
    • zgc:56396 antibody
    see all

图片

  • ab87275 at 1/250 dilution staining MTA3 in Human breast carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining
  • Anti-MTA3 antibody (ab87275) at 1/2000 dilution + Human trophoblast cell lysate at 10 µg

    Secondary
    HRP-conjugated donkey anti-rabbit polyclonal IgG at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 67 kDa


    Exposure time : 2 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab87275 at 1/250 dilution staining MTA3 in Human seminoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining

文献

This product has been referenced in:
  • Paget S  et al. HIC1 (hypermethylated in cancer 1) SUMOylation is dispensable for DNA repair but is essential for the apoptotic DNA damage response (DDR) to irreparable DNA double-strand breaks (DSBs). Oncotarget 8:2916-2935 (2017). WB . Read more (PubMed: 27935866) »
  • Dubuissez M  et al. Protein Kinase C-Mediated Phosphorylation of BCL11B at Serine 2 Negatively Regulates Its Interaction with NuRD Complexes during CD4+ T-Cell Activation. Mol Cell Biol 36:1881-98 (2016). Read more (PubMed: 27161321) »

See all 3 Publications for this product

客户评价及客户问答

Abreviews
Application
ChIP
Sample
Human Cell lysate - nuclear (Jurkat cells)
Negative control
ChIP with rabbit IgG qPCR on GAPDH promoter
Specification
Jurkat cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldéhyde
Username

Dr. Marion Dubuissez

Verified customer

提交于 Nov 03 2015

Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (MOLT4 cells)
Total protein in input
10 µg
Immuno-precipitation step
Protein A/G
Specification
MOLT4 cells
Username

Dr. Marion Dubuissez

Verified customer

提交于 Aug 24 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (trophoblast cells)
Loading amount
10 µg
Specification
trophoblast cells
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
0.5% casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Aug 01 2012

Thank you very much for sending the order number. I've created the discount code below, which can be used to receive a free primary antibody after submitting the review of ab87275.

DISCOUNT CODE: ***
Expiration date: December 2, 2012
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Thank you very much for your reply and for sending the additional information about your assay.

I agree that the results with the two vials of ab87275 are different, but since these vials contained antibody from the same production batch, thi...

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Thank you very much for contacting us and letting us know about this issue.

These two lots, GR38863-2 and GR38863-1, are actually from the same production batch so we would expect to see pretty similar results using both of them. Are there an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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