概述

  • 产品名称Anti-MTA2抗体
    参阅全部 MTA2 一抗
  • 描述
    兔多克隆抗体to MTA2
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Cow, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human MTA2.

    (Peptide available as ab98903.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293; Caco2; MCF7; Raji; PC12.

性能

应用

Our Abpromise guarantee covers the use of ab85872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 75 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 相关性The p53 tumor suppressor gene integrates numerous signals that control cell life and death. There are several proteins that are involved in the p53 pathway, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and MTA2. The transcriptional activity of p53 is modulated by protein stability and acetylation. MTA2, also termed MTA1L1, was found to be a subunit of nucleosome remodeling and deacetylating (NuRD) complex. MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by MTA2 represses p53 dependent transcriptional activation and modulates p53 mediated cell growth arrest and apoptosis. MTA2 is ubiquitously expressed in human tissues.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • DKFZp686F2281 antibody
    • Mata1l1 antibody
    • Metastasis associated 1 family member 2 antibody
    • Metastasis associated 1 like 1 antibody
    • Metastasis associated gene 1 like 1 antibody
    • Metastasis associated gene family member 2 antibody
    • Metastasis associated protein 2 antibody
    • Metastasis associated protein MTA 2 antibody
    • Metastasis associated protein MTA2 antibody
    • Mmta2 antibody
    • MTA1 L1 protein antibody
    • MTA1L1 antibody
    • p53 target protein in deacetylase complex antibody
    • PID antibody
    see all

Anti-MTA2 antibody 图像

  • All lanes : Anti-MTA2 antibody (ab85872) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 77 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 49 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • ICC/IF image of ab74489 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85872, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa and Hek293 cells at 5µg/ml.
  • IHC image of MTA2 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85872, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Anti-MTA2 antibody (ab85872) at 1 µg/ml + PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 75 kDa
    Observed band size : 77 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa,55 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

Anti-MTA2 antibody (ab85872)参考文献

ab85872 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab85872.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"