概述

  • 产品名称Anti-MTA1抗体
    参阅全部 MTA1 一抗
  • 描述
    兔多克隆抗体to MTA1
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • 免疫原

    A synthetic peptide corresponding to a region between residue 665 and the C-terminus (residue 715) of MTA1 (NP_004680.1).

  • 阳性对照
    • Whole cell lysate from Hela cells, NIH3T3 cells and 293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab71153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 35, 81 kDa (predicted molecular weight: 81 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能May be involved in the regulation of gene expression by covalent modification of histone proteins. Isoform Long is a corepressor of estrogen receptor (ER). Isoform Short binds to ER and sequesters it in the cytoplasm and enhances non-genomic responses of ER.
  • 组织特异性Widely expressed. High expression in brain, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual.
  • 序列相似性Contains 1 BAH domain.
    Contains 1 ELM2 domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 SANT domain.
  • 发展阶段Highly expressed in metastatic cells.
  • 结构域Isoform Short contains a Leu-Arg-Ile-Leu-Leu motif (ER binding motif).
  • 细胞定位Cytoplasm and Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Metastasis associated 1 antibody
    • Metastasis associated gene 1 antibody
    • Metastasis associated gene 1 protein antibody
    • Metastasis associated protein antibody
    • Metastasis associated protein MTA 1 antibody
    • Metastasis associated protein MTA1 antibody
    • Metastasis-associated protein MTA1 antibody
    • MTA 1 antibody
    • MTA1 antibody
    • MTA1_HUMAN antibody
    see all

Anti-MTA1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling MTA1 with ab71153 at 1/1000 (0.2 µg/ml). Detection: DAB.
  • All lanes : Anti-MTA1 antibody (ab71153) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


    Predicted band size : 81 kDa
    Observed band size : 81 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation/ Western Blot of MTA1
    Lane 1: ab71153 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Subsequent WB detection was performed using 1 µg/ml ab71153.
    Chemiluminescence with an exposure time of 1 second.
  • ICC/IF image of ab71153 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MTA1 antibody (ab71153)参考文献

ab71153 has not yet been referenced specifically in any publications.

Product Wall

Application ChIP
Sample Human Cell lysate - nuclear (Jurkat cells)
Negative control ChIP with rabbit IgG qPCR on GAPDH promoter
Specification Jurkat cells
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Username

Dr. Marion Dubuissez

Verified customer

提交于 Nov 03 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Treatment 10uM T3
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Sep 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Treatment 10uM T3 for 24 hrs
Total protein in input 100 µg
Username

Abcam user community

Verified customer

提交于 Jun 26 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Treatment 10uM T3 for 24 hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (MCF7 cells)
Total protein in input 1 µg
Specification MCF7 cells
Treatment 10uM T3 for 24 hrs
Immuno-precipitation step Protein A/G
Username

Mr. Sanjib Chaudhary

Verified customer

提交于 Aug 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"