Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon and Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Specification
Colon and Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/750
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
This Ab appears to give specific immunolabelling of mitochondria.
Submitted composite image is of kidney medullary region ( upper) and colon ( lower).
This Ab can easily be diluted further as cells containing an abundance of mitochondria are overstained but, cells containing relatively few mitochondria will be more difficult to discern.
Of course, one would need to ascertain the validity of my results by comparing this positive pattern with another anti Mitochondria Ab that has been validated for FFPW sections.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Dec 13 2010