Anti-MT-CYB抗体(ab81215)

概述

  • 产品名称Anti-MT-CYB抗体
    参阅全部 MT-CYB 一抗
  • 描述
    兔多克隆抗体to MT-CYB
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cat
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (TPMRKINPLM KLINHSFIDL PTPSNISAWW NFGSLLGACL ILQITTGLFL) of Human MT-CYB (NP_536855).

  • 阳性对照
    • HeLa cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab81215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

靶标

  • 功能Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
  • 疾病相关Defects in MT-CYB are a rare cause of mitochondrial dysfunction underlying different myopathies. They include mitochondrial encephalomyopathy, hypertrophic cardiomyopathy (HCM), and sporadic mitochondrial myopathy (MM). In mitochondrial myopathy, exercise intolerance is the predominant symptom. Additional features include lactic acidosis, muscle weakness and/or myoglobinuria. Defects in MTCYB are also found in cases of exercise intolerance accompanied by deafness, mental retardation, retinitis pigmentosa, cataract, growth retardation, epilepsy (multisystem disorder).
    Cardiomyopathy, infantile histiocytoid
    Leber hereditary optic neuropathy
  • 序列相似性Belongs to the cytochrome b family.
  • 细胞定位Mitochondrion inner membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COB antibody
    • Complex III subunit 3 antibody
    • Complex III subunit III antibody
    • CYB_HUMAN antibody
    • CYTB antibody
    • Cytochrome b antibody
    • Cytochrome b c1 complex subunit 3 antibody
    • Cytochrome b-c1 complex subunit 3 antibody
    • Mitochondrially encoded cytochrome b antibody
    • MT-CYB antibody
    • MTCYB antibody
    • Ubiquinol cytochrome c reductase complex cytochrome b subunit antibody
    • Ubiquinol-cytochrome-c reductase complex cytochrome b subunit antibody
    see all

Anti-MT-CYB antibody 图像

  • Anti-MT-CYB antibody (ab81215) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa

Anti-MT-CYB antibody (ab81215)参考文献

ab81215 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HeLa wt vs HeLa rho-0)
Loading amount 70 µg
Specification HeLa wt vs HeLa rho-0
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jul 02 2012

Thanks for your patience.  I have place the order for ab103405 (order#******), you should hopefully have it by the 20th Oct. If there is anything else I can do, just give me a shout. You have a great weekend too.

Thank you forcontacting Abcam about ab81215, anti-MT-CYB antibody I am sorry to hear that you are experiencing problems with this antibody.  It is covered under our Abpromise, which guarantees that it will work on human tissue in western blot. I kn...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"