概述

  • 产品名称Anti-MST3抗体
    参阅全部 MST3 一抗
  • 描述
    兔多克隆抗体to MST3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide containing a sequence corresponding to a region within amino acids 125-189 (LREILKGLDY LHSEKKIHRD IKAANVLLSE HGEVKLADFG VAGQLTDTQI KRNTFVGTPF WMAPE) of Human MST3 (NP_001027467).

  • 阳性对照
    • Raji whole cell lysate; HeLa cells; 293T, A431 and H1299 cell lines

性能

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应用

Our Abpromise guarantee covers the use of ab96705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve.
  • 组织特异性Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.
    Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues.
  • 细胞定位Cytoplasm. Nucleus. Membrane. The truncated form (MST3/N) translocates to the nucleus. Co-localizes with STK38L in the membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • epididymis secretory protein Li 95 antibody
    • HEL-S-95 antibody
    • Mammalian STE20 like protein kinase 3 antibody
    • Mammalian STE20-like protein kinase 3 antibody
    • Mammalian STE20-like protein kinase 3 C-terminal antibody
    • Mammalian STE20-like protein kinase 3 N-terminal antibody
    • Mammalian sterile 20-like 3 antibody
    • MST-3 antibody
    • MST3/C antibody
    • MST3/N antibody
    • MST3B antibody
    • serine threonine kinase 24 antibody
    • Serine/threonine kinase 24 (Ste20, yeast homolog) antibody
    • Serine/threonine-protein kinase 24 12 kDa subunit antibody
    • Serine/threonine-protein kinase 24 antibody
    • STE20 homolog yeast antibody
    • STE20 like kinase MST3 antibody
    • STE20-like kinase MST3 antibody
    • Stk24 antibody
    • STK24_HUMAN antibody
    • STK3 antibody
    see all

Anti-MST3 antibody 图像

  • Anti-MST3 antibody (ab96705) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 48 kDa
  • Immunofluorescence analysis of MST3 in paraformaldehyde-fixed HeLa cells, using ab96705 at 1/200 dilution (top image) and merged with DNA probe (bottom image).

Anti-MST3 antibody (ab96705)参考文献

ab96705 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (Mouse cortex at E16)
Specification Mouse cortex at E16
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
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提交于 Oct 07 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA and goat serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (Mouse cortical neuron)
Specification Mouse cortical neuron
Permeabilization Yes - 0.1% Triton X-100
Fixative Formaldehyde
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提交于 Oct 07 2013

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