Anti-MST1/MST2 (phospho T183)抗体(ab79199)


  • 产品名称
    Anti-MST1/MST2 (phospho T183)抗体
    参阅全部 MST1/MST2 一抗
  • 描述
    兔多克隆抗体to MST1/MST2 (phospho T183)
  • 特异性
    ab79199 detects endogenous levels of MST1/MST2 only when phosphorylated at threonine 183.
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human MST1/MST2 around the phosphorylation site of threonine 183 (R-N-TP-V-I).

  • 阳性对照
    • Human liver carcinoma tissue. Extracts from 293 cells treated with H2O2 (100uM, 15mins).


  • 形式
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab79199 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab79199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.


  • 相关性
    The human serine/threonine kinase, mammalian STE20 like kinase (MST), is considerably homologous to the budding yeast kinases, SPS1 and STE20, throughout their kinase domains. When stably expressed in HeLa cells, MST highly sensitizes the cells to death receptor mediated apoptosis by accelerating caspase 3 activation. These findings suggest that MST1 and MST2 play a role in apoptosis both upstream and downstream of caspase activation. MST1 is cleaved and activated by caspases during apoptosis and is capable of inducing apoptotic morphological changes such as chromatin condensation upon overexpression. Mammalian Sterile 20 Like 2 (MST2) is most similar to the previously identified MST1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in vitro kinase assay indicates that MST2 can phosphorylate an exogenous substrate, as well as itself, and phospho amino acid analysis indicates that it is a serine/threonine protein kinase.
  • 细胞定位
    Cytoplasm. Nucleus. Note: The caspase cleaved form cycles between the nucleus and cytoplasm.
  • 数据库链接
  • 别名
    • DKFZp686A2068 antibody
    • FLJ90748 antibody
    • KRS1 antibody
    • KRS2 antibody
    • Mammalian STE20 like protein kinase 1 antibody
    • Mammalian STE20 like protein kinase 2 antibody
    • MST 1 antibody
    • MST 2 antibody
    • MST1 antibody
    • MST2 antibody
    • Serine/threonine kinase 3 (STE20 homolog yeast) antibody
    • Serine/threonine kinase 3 (Ste20 yeast homolog) antibody
    • Serine/threonine kinase 3 antibody
    • Serine/threonine kinase 4 antibody
    • Serine/threonine protein kinase 3 antibody
    • Serine/threonine protein kinase 4 antibody
    • Serine/threonine protein kinase Krs 1 antibody
    • Serine/threonine protein kinase Krs 2 antibody
    • STE20 like kinase MST1 antibody
    • STE20 like kinase MST2 antibody
    • STK 3 antibody
    • STK 4 antibody
    • STK3 antibody
    • STK4 antibody
    • YSK3 antibody
    see all

Anti-MST1/MST2 (phospho T183) antibody 图像

  • All lanes : Anti-MST1/MST2 (phospho T183) antibody (ab79199)

    Lane 1 : Extracts from 293 cells, treated with H2O2 (100uM, 15mins)
    Lane 2 : Extracts from 293 cells, treated with H2O2 (100uM, 15mins) with immunising phosphopeptide

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • ab79199, at 1/50 dilution, staining MST1/MST2 in human liver carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.

Anti-MST1/MST2 (phospho T183) antibody (ab79199)参考文献

This product has been referenced in:
  • Dixit D  et al. Chaetocin-induced ROS-mediated apoptosis involves ATM-YAP1 axis and JNK-dependent inhibition of glucose metabolism. Cell Death Dis 5:e1212 (2014). WB ; Human . Read more (PubMed: 24810048) »

See 1 Publication for this product

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Since ab79199 has not been tried on mouse samples, we do not guarantee it for this species. The guarantee applies for tested species and applications only.

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I am sorry to hear that the antibodies are not working as expected.

Your protocol looks fine to me. I wanted to ask you a few more questions:

1) Both antibodies detect a phospho-site. Under the expe...

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