Anti-MSK2 / RSK-B抗体(ab99411)
Key features and details
- Rabbit polyclonal to MSK2 / RSK-B
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-MSK2 / RSK-B抗体
参阅全部 MSK2 / RSK-B 一抗 -
描述
兔多克隆抗体to MSK2 / RSK-B -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human MSK2/ RSK-B aa 700-800. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_003933.1
Database link: O75676 -
阳性对照
- HeLa, 293T or NIH 3T3 cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99411于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 86 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 86 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) and result in the transcriptional activation of several immediate early genes, including proto-oncogenes FOS and JUN (By similarity). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14). -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8986 Human
- Entrez Gene: 56613 Mouse
- Omim: 603606 Human
- SwissProt: O75676 Human
- SwissProt: Q9Z2B9 Mouse
- Unigene: 105584 Human
- Unigene: 20914 Mouse
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别名
- 90 kDa ribosomal protein S6 kinase 4 antibody
- EC 2.7.11.1 antibody
- KS6A4_HUMAN antibody
see all
图片
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All lanes : Anti-MSK2 / RSK-B antibody (ab99411) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MSK2 / RSK-B with ab99411 at 1/200 (1µg/mg). Detection: DAB.
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Detection of MSK2 / RSK-B in immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded on gel) using ab99411 at 3 µg/mg lysate for IP, and at 1 µg/ml for the subsequent western blot.
Detection: chemiluminescence with an exposure time of 30 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab99411 被引用在 2 文献中.
- Zhang C et al. Sulforaphene induces apoptosis and inhibits the invasion of esophageal cancer cells through MSK2/CREB/Bcl-2 and cadherin pathway in vivo and in vitro. Cancer Cell Int 19:342 (2019). PubMed: 31889894
- Khan P et al. Mitogen- and stress-activated protein kinases 1 and 2 are required for maximal trefoil factor 1 induction. PLoS One 8:e63189 (2013). PubMed: 23675462