RabMAb

Anti-MSK1 (phospho S376)抗体[E375] (ab32190)

概述

  • 产品名称Anti-MSK1 (phospho S376)抗体[E375]
    参阅全部 MSK1 一抗
  • 描述
    兔单克隆抗体[E375] to MSK1 (phospho S376)
  • 特异性This antibody will detect MSK1 phosphorylation on Serine 376.
  • 经测试应用适用于: WB, ICC, IP, IHC-Pmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MSK1.

  • 阳性对照
    • WB: K562 cell lysate. IHC-P Human urinary bladder carcinoma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-MSK1 (phospho S376) antibody (Alexa Fluor® 647) [E375] (ab201263)

    Anti-MSK1 (phospho S376) antibody (Alexa Fluor® 488) [E375] (ab201759)

性能

应用

Our Abpromise guarantee covers the use of ab32190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 90 kDa).
ICC 1/100 - 1/250.
IP 1/30.
IHC-P Use at an assay dependent concentration.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
    • 组织特异性Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
    • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 2 protein kinase domains.
    • 翻译后修饰Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.
    • 细胞定位Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 90 kDa ribosomal protein S6 kinase 5 antibody
      • EC 2.7.11.1 antibody
      • KS6A5_HUMAN antibody
      • MGC1911 antibody
      • Mitogen and stress activated protein kinase 1 antibody
      • MSPK1 antibody
      • Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
      • Nuclear mitogen- and stress-activated protein kinase 1 antibody
      • Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
      • Ribosomal protein S6 kinase 90kDa antibody
      • Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
      • Ribosomal Protein S6 Kinase Alpha 5 antibody
      • Ribosomal protein S6 kinase alpha-5 antibody
      • RLPK antibody
      • RPS6KA5 antibody
      • RSK Like Protein Kinase antibody
      • RSK-like protein kinase antibody
      • RSKL antibody
      • S6K alpha 5 antibody
      • S6K-alpha-5 antibody
      see all

    Anti-MSK1 (phospho S376) antibody [E375] 图像

    • All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution

      Lane 1 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates
      Lane 2 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates with MSK1 (phosphor S376) peptide
      Lane 3 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates with MSK1 non-phospho peptide

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 90 kDa


      Exposure time : 10 seconds

      Blocking/Diluting buffer: 5% NFDM/TBST

      Observed band: 90, 80 kDa.
      The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.

    • All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution

      Lane 1 : Untreated HEK293 (human embryonic kidney) whole cell lysates
      Lane 2 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysates
      Lane 3 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 90 kDa


      Exposure time : 5 seconds

      Blocking/Diluting buffer 5% NFDM/TBST.

      Observed band: 90, 80 kDa.
      The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.

       

    • All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/5000 dilution

      Lane 1 : Untreated K562 cell lysate
      Lane 2 : K562 cell lysate treated with Alkaline


      Predicted band size : 90 kDa
      Observed band size : 87 kDa (why is the actual band size different from the predicted?)
    • Ab32190, at a dilution of 1/50, staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry.

    Anti-MSK1 (phospho S376) antibody [E375] (ab32190)参考文献

    This product has been referenced in:
    • Khan SA  et al. p38-MAPK/MSK1-mediated overexpression of histone H3 serine 10 phosphorylation defines distance-dependent prognostic value of negative resection margin in gastric cancer. Clin Epigenetics 8:88 (2016). Read more (PubMed: 27588146) »
    • Rylander D  et al. Pharmacological modulation of glutamate transmission in a rat model of L-DOPA-induced dyskinesia: effects on motor behavior and striatal nuclear signaling. J Pharmacol Exp Ther 330:227-35 (2009). Read more (PubMed: 19357321) »

    See all 3 Publications for this product

    Product Wall

    Thank you for your response.
    This is to let you know that I have placed a new order for you - for one vial of ab32190 as a free of charge replacement and the new item has been added to order number is 1095220.
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    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.
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    Thank you for your response and your patience.
    This antibody has been tested for Western blot application on K562 cell lysate. It may well be that the levels of MSK1 (phospho S376) is different in Wrl68 cells and higher concentration of this primar...

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    MY colleague Dr.XXXX is out of office today. She will follow this case next week Monday.
    Many thanks for having patience!

    Thank you for your enquiry regarding ab32190 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.
    As our Abpromise indicates, in the event that...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Epithelial, neutrophil, monocyte)
    Loading amount 500000 cells
    Specification Epithelial, neutrophil, monocyte
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    提交于 Sep 02 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"