重组Anti-MSK1 (phospho S376)抗体[E375] (ab32190)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E375] to MSK1 (phospho S376)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MSK1 (phospho S376)抗体[E375]
参阅全部 MSK1 一抗 -
描述
兔单克隆抗体[E375] to MSK1 (phospho S376) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: K562 cell lysate. IHC-P Human urinary bladder carcinoma. ICC/IF: HepG2 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E375 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32190于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 90 kDa).
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IHC-P | (5) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/500.
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说明 |
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WB
1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 90 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/500. |
靶标
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功能
Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14). -
组织特异性
Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
翻译后修饰
Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. -
细胞定位
Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic. - Information by UniProt
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数据库链接
- Entrez Gene: 9252 Human
- Entrez Gene: 314384 Rat
- Omim: 603607 Human
- SwissProt: O75582 Human
- Unigene: 510225 Human
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别名
- 90 kDa ribosomal protein S6 kinase 5 antibody
- EC 2.7.11.1 antibody
- KS6A5_HUMAN antibody
see all
图片
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All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution
Lane 1 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates
Lane 2 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates with MSK1 (phosphor S376) peptide
Lane 3 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates with MSK1 non-phospho peptide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Exposure time: 10 secondsBlocking/Diluting buffer: 5% NFDM/TBST
Observed band: 90, 80 kDa.
The 80 kDa band may represent isoform 2 or isoform 3 of MSK1. -
Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling MSK1 (phospho S376) with ab32190 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as Nuclear counterstain.Confocal image showing decreased nuclear staining in HepG2 cells treated with Alkaline Phosphatase (37℃ 1h).
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All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution
Lane 1 : Untreated HEK293 (human embryonic kidney) whole cell lysate
Lane 2 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate
Lane 3 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate. Then the membrane was incubated with Alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Exposure time: 5 secondsBlocking/Diluting buffer 5% NFDM/TBST.
Observed band: 90, 80 kDa.
The 80 kDa band may represent isoform 2 or isoform 3 of MSK1. -
All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/5000 dilution
Lane 1 : Untreated K562 cell lysate
Lane 2 : K562 cell lysate treated with Alkaline
Predicted band size: 90 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted? -
Ab32190, at a dilution of 1/50, staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab32190 被引用在 4 文献中.
- Li J et al. Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase. Nat Commun 12:896 (2021). PubMed: 33563994
- Khan SA et al. p38-MAPK/MSK1-mediated overexpression of histone H3 serine 10 phosphorylation defines distance-dependent prognostic value of negative resection margin in gastric cancer. Clin Epigenetics 8:88 (2016). PubMed: 27588146
- Rylander D et al. Pharmacological modulation of glutamate transmission in a rat model of L-DOPA-induced dyskinesia: effects on motor behavior and striatal nuclear signaling. J Pharmacol Exp Ther 330:227-35 (2009). PubMed: 19357321
- Westin JE et al. Spatiotemporal pattern of striatal ERK1/2 phosphorylation in a rat model of L-DOPA-induced dyskinesia and the role of dopamine D1 receptors. Biol Psychiatry 62:800-10 (2007). IHC ; Rat . PubMed: 17662258