概述

  • 产品名称Anti-mSin3A抗体- ChIP Grade
    参阅全部 mSin3A 一抗
  • 描述
    兔多克隆抗体to mSin3A - ChIP Grade
  • 经测试应用适用于: ICC/IF, ChIP, CHIPseq, IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse mSin3A aa 1-19.
    Sequence:

    MKRRLDDQESDVYAAQQRR


    (Peptide available as ab4995)

性能

应用

Our Abpromise guarantee covers the use of ab3479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50 - 1/500.
ChIP Use at an assay dependent concentration.
CHIPseq Use at an assay dependent concentration. PubMed: 21490601
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 145 kDa).Can be blocked with mSin3A peptide (ab4995).
IHC-P Use a concentration of 2 µg/ml.

靶标

  • 功能Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3A to DNA. Acts as a corepressor for REST.
  • 序列相似性Contains 3 PAH (paired amphipathic helix) domains.
  • 翻译后修饰SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2.
  • 细胞定位Nucleus. Nucleus > nucleolus. Recruited to the nucleolus by SAP30L.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AW553200 antibody
    • DKFZP434K2235 antibody
    • FLJ90319 antibody
    • Histone deacetylase complex subunit Sin 3a antibody
    • Histone deacetylase complex subunit Sin3a antibody
    • KIAA0700 antibody
    • Kiaa4126 antibody
    • mKIAA4126 antibody
    • Paired amphipathic helix protein Sin 3a antibody
    • Paired amphipathic helix protein Sin3a antibody
    • Sin 3a antibody
    • SIN3 homolog A antibody
    • SIN3 homolog A transcription regulator (yeast) antibody
    • SIN3 homolog A transcription regulator antibody
    • SIN3 transcription regulator homolog A antibody
    • Sin3a antibody
    • SIN3A protein antibody
    • SIN3A_HUMAN antibody
    • Transcriptional co repressor Sin 3A antibody
    • Transcriptional co repressor Sin3A antibody
    • Transcriptional corepressor Sin3a antibody
    • Transcriptional regulator SIN3A antibody
    see all

Anti-mSin3A antibody - ChIP Grade 图像

  • Immunocytochemical immunoflurescence analysis of NIH-3T3 cells labelling mSin3A using ab3479. Formalin-fixed cells were permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were then incubated with ab3479 in 3% BSA-PBS at a dilution of 1:100 overnight in a 4°C high humidity environment. Cells were then washed with PBST and incubated with a green DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Cells were counterstained with DAPI or Hoechst labelling the nuclear DNA blue. The Left Image is a negative control without the prescence of ab3479. Image magnification is 60X.

  • Immunocytochemical immunoflurescence analysis of HeLa cells labelling mSin3A using ab3479. Formalin-fixed cells were permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were then incubated with ab3479 in 3% BSA-PBS at a dilution of 1:200 overnight in a 4°C high humidity environment. Cells were then washed with PBST and incubated with a green DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Cells were counterstained blue with DAPI or Hoechst labelling the nuclear DNA and red against actin using an Alexa Fluor® 554 conjugate. The Left Image is a negative control without the prescence of ab3479. Image magnification is 60X.

  • Immunocytochemical immunoflurescence analysis of NIH-3T3 cells labelling mSin3A using ab3479. Formalin-fixed cells were permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were then incubated with ab3479 in 3% BSA-PBS at a dilution of 1:100 overnight in a 4°C high humidity environment. Cells were then washed with PBST and incubated with a green DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Cells were counterstained blue with DAPI or Hoechst labelling the nuclear DNA and red against actin using an Alexa Fluor® 554 conjugate. The Left Image is a negative control without the prescence of ab3479. Image magnification is 60X.



  • Predicted band size : 145 kDa
    Western blot of mSin3A on K562 cell extract using ab3479.
  • ab3479 (2µg/ml) staining mSin3A in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclei of epithelial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1/ EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-mSin3A antibody - ChIP Grade (ab3479)参考文献

This product has been referenced in:
  • Heckman LD  et al. Rett-causing mutations reveal two domains critical for MeCP2 function and for toxicity in MECP2 duplication syndrome mice. Elife 3:N/A (2014). WB ; Mouse . Read more (PubMed: 24970834) »
  • Lit LC  et al. LATS2 is a modulator of estrogen receptor alpha. Anticancer Res 33:53-63 (2013). Read more (PubMed: 23267128) »

See all 11 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - nuclear (Embryonic stem cell)
Gel Running Conditions Non-reduced Denaturing (7%)
Loading amount 60 µg
Specification Embryonic stem cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Aug 19 2016

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (7%gel)
Sample Mouse Tissue lysate - whole (hippocampus)
Specification hippocampus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Mar 30 2015

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Application Immunoprecipitation
Immuno-precipitation step Staph. aureus
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Total protein in input 100 µg
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提交于 Sep 09 2013

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Application ChIP
Detection step Semiquantitative PCR
Sample Human Cell lysate - nuclear (MCF7)
Specification MCF7
Negative control IgG processed sample
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: FORMALDEHYDE 10%
Positive control INPUT processes with out antibody and decrosslinked precipitated DNA
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提交于 Sep 09 2013

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Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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提交于 Sep 05 2013

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Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (HEK293T)
Specification HEK293T
Total protein in input 40 µg
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提交于 Jul 05 2013

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Application ChIP
Detection step Semiquantitative PCR
Sample Human Cell lysate - nuclear (MCF7)
Specification MCF7
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: FORMALDEHYDE 10%
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提交于 Jul 05 2013

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Sample Mouse Tissue sections (BREAST CANCER TISSUE)
Specification BREAST CANCER TISSUE
Permeabilization Yes - xylene
Fixative Formaldehyde
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提交于 Jul 05 2013

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Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing (8)
Sample Human Cell lysate - whole cell (HEK293T)
Specification HEK293T
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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提交于 Jul 04 2013

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH/3T3)
Specification NIH/3T3
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 21°C
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Mrs. Filomena Ricciardi

Verified customer

提交于 Jun 26 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"