产品名称 Anti-mSin3A抗体 参阅全部 mSin3A 一抗
IHC-P, WB more details
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (
KRRLDDQESPVYAAQQRRIPGSTEAFPHQHRVLAPAPPVYEAVSETMQSA ) of Human mSin3A (NP_056292).
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
存储溶液 Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Predicted molecular weight: 145 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能 Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3A to DNA. Acts as a corepressor for REST.
序列相似性 Contains 3 PAH (paired amphipathic helix) domains.
翻译后修饰 SUMO1 sumoylated by TOPORS. Probably desumoylated by SENP2.
细胞定位 Nucleus. Nucleus > nucleolus. Recruited to the nucleolus by SAP30L.
Information by UniProt
Anti-mSin3A antibody 图像
Western blot - mSin3A antibody (ab83292)
Anti-mSin3A antibody (ab83292) at 1 µg/ml + HeLa cell lysate at 10 µg Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 145 kDa Observed band size : 144 kDa ( why is the actual band size different from the predicted?) Additional bands at : 88 kDa,<40 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-mSin3A antibody(ab83292)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling mSin3A with ab83292 at 5.0µg/ml.
Anti-mSin3A antibody (ab83292)参考文献
has not yet been referenced specifically in any publications.
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