产品名称 Anti-MSI2抗体 参阅全部 MSI2 一抗
ICC/IF, WB more details
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse MSI2.
This antibody gave a positive signal in U87MG whole cell lysate as well as both Mouse Brain Mouse Spinal cord tissue lysates.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
功能 RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
组织特异性 Ubiquitous; detected at low levels.
疾病相关 Note=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
序列相似性 Belongs to the Musashi family. Contains 2 RRM (RNA recognition motif) domains.
细胞定位 Cytoplasm. Associated with polysomes.
Information by UniProt
Anti-MSI2 antibody 图像
Western blot - Anti-MSI2 antibody (ab105889)
All lanes : Anti-MSI2 antibody (ab105889) at 1 µg/ml Lane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Spinal Cord (Mouse) Tissue Lysate Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 37 kDa Observed band size : 37 kDa Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands. Exposure time : 20 minutes
Immunocytochemistry/ Immunofluorescence - Anti-MSI2 antibody (ab105889)
ICC/IF image of ab105889 stained Mouse Embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab105889 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (
ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-MSI2 antibody (ab105889)参考文献
has not yet been referenced specifically in any publications.
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