Anti-MSH6抗体[44], prediluted (ab59755)


  • 产品名称Anti-MSH6抗体[44], prediluted
    参阅全部 MSH6 一抗
  • 描述
    小鼠单克隆抗体[44] to MSH6, prediluted
  • 经测试应用适用于: IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
  • 免疫原

    Synthetic Human MSH6 peptide

  • 阳性对照
    • Tonsil, colon carcinoma tissue.



Our Abpromise guarantee covers the use of ab59755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明IHC-P: This antibody has been pretitered.
    IHC-Fr: This antibody has been pretitered.

    This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

    We suggest an incubation period of 30 to 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.

    Not yet tested in other applications.
  • 靶标

    • 功能Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair.
    • 疾病相关Defects in MSH6 are the cause of hereditary non-polyposis colorectal cancer type 5 (HNPCC5) [MIM:600678]. Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. MSH6 mutations appear to be associated with atypical HNPCC and in particular with development of endometrial carcinoma or atypical endometrial hyperplasia, the presumed precursor of endometrial cancer. Defects in MSH6 are also found in familial colorectal cancers (suspected or incomplete HNPCC) that do not fulfill the Amsterdam criteria for HNPCC.
      Defects in MSH6 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    • 序列相似性Belongs to the DNA mismatch repair mutS family.
      Contains 1 PWWP domain.
    • 翻译后修饰The N-terminus is blocked.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA mismatch repair protein Msh6 antibody
      • G/T mismatch binding protein antibody
      • G/T mismatch-binding protein antibody
      • GTBP antibody
      • GTMBP antibody
      • hMSH6 antibody
      • HNPCC 5 antibody
      • HNPCC5 antibody
      • HSAP antibody
      • MSH 6 antibody
      • MSH6 antibody
      • MSH6_HUMAN antibody
      • mutS (E. coli) homolog 6 antibody
      • MutS alpha 160 kDa subunit antibody
      • MutS homolog 6 (E. coli) antibody
      • mutS homolog 6 antibody
      • MutS-alpha 160 kDa subunit antibody
      • p160 antibody
      • Sperm associated protein antibody
      see all

    Anti-MSH6 antibody [44], prediluted 图像

    • Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil tissue sections, labelling MSH6 with ab59755.

    Anti-MSH6 antibody [44], prediluted (ab59755)参考文献

    ab59755 has not yet been referenced specifically in any publications.

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