概述

  • 产品名称
  • 描述
    兔多克隆抗体to MSH4
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    A synthetic peptide corresponding to C terminal residues of Mouse MSH4

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 19247432
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa.
ELISA Use at an assay dependent concentration.

靶标

  • 功能
    Involved in meiotic recombination. Required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis.
  • 组织特异性
    Testis and ovary specific.
  • 序列相似性
    Belongs to the DNA mismatch repair MutS family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hMSH4 antibody
    • MSH 4 antibody
    • MSH4 antibody
    • MSH4_HUMAN antibody
    • MutS homolog 4 (E. Coli) antibody
    • MutS homolog 4 antibody
    • MutS protein homolog 4 antibody
    see all

Anti-MSH4 antibody 图像

  • ab58666 staining MSH4 in mouse spermatocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 1% paraformaldehyde and 0.15% Triton X-100. An Alexa flour conjugated anti rabbit IgG was used as secondary. Green colour staining was observed with ab58666.

Anti-MSH4 antibody (ab58666)参考文献

This product has been referenced in:
  • Rong M  et al. Meiotic cohesin subunits RAD21L and REC8 are positioned at distinct regions between lateral elements and transverse filaments in the synaptonemal complex of mouse spermatocytes. J Reprod Dev 62:623-630 (2016). IF ; Mouse . Read more (PubMed: 27665783) »
  • Holloway JK  et al. Mammalian CNTD1 is critical for meiotic crossover maturation and deselection of excess precrossover sites. J Cell Biol 205:633-41 (2014). Read more (PubMed: 24891606) »

See all 8 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (spermatocytes)
Permeabilization
No
Specification
spermatocytes
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative
Formaldehyde
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提交于 Jun 01 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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