概述
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产品名称Anti-MSH3抗体
参阅全部 MSH3 一抗 -
描述兔多克隆抗体to MSH3
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宿主Rabbit
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经测试应用适用于: IHC-P, ICC/IFmore details
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种属反应性与反应: Human
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免疫原
A synthetic peptide derived from an internal region of Human MSH3 protein.
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阳性对照
- Human rectal carcinoma tissue
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C.
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存储溶液Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明Immunogen affinity purified immunoglobulin from rabbit serum.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab93663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
靶标
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功能Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
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疾病相关Defects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
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序列相似性Belongs to the DNA mismatch repair mutS family. MSH3 subfamily.
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翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
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数据库链接
- Entrez Gene: 4437 Human
- Omim: 600887 Human
- SwissProt: P20585 Human
- Unigene: 280987 Human
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别名
- Divergent upstream protein antibody
- DNA mismatch repair protein antibody
- DNA mismatch repair protein Msh 3 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH3 antibody (ab93663)ab93663, at a 1/100 dilution, staining MSH3 in formalin fixed, paraffin embedded Human rectal carcinoma tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-MSH3 antibody (ab93663)ICC/IF image of ab93663 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93663, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
文献
ab93663 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"