概述

  • 产品名称Anti-MSH3抗体
    参阅全部 MSH3 一抗
  • 描述
    兔多克隆抗体to MSH3
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from an internal region of human MSH3.

  • 阳性对照
    • Extracts from HUVEC cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
ELISA 1/20000.

靶标

  • 功能Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
  • 疾病相关Defects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
  • 序列相似性Belongs to the DNA mismatch repair mutS family. MSH3 subfamily.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Divergent upstream protein antibody
    • DNA mismatch repair protein antibody
    • DNA mismatch repair protein Msh 3 antibody
    • DNA mismatch repair protein MSH3 antibody
    • DUC 1 antibody
    • DUC1 antibody
    • DUG antibody
    • DUP antibody
    • hMSH3 antibody
    • MGC163306 antibody
    • MGC163308 antibody
    • Mismatch repair protein 1 antibody
    • MRP 1 antibody
    • MRP1 antibody
    • MSH 3 antibody
    • MSH3 antibody
    • MSH3_HUMAN antibody
    • MutS homolog 3 (E. coli) antibody
    • MutS homolog 3 antibody
    see all

Anti-MSH3 antibody 图像

  • All lanes : Anti-MSH3 antibody (ab69619) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 127 kDa
    Observed band size : 127 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

Anti-MSH3 antibody (ab69619)参考文献

This product has been referenced in:
  • Zhao J  et al. Mismatch repair and nucleotide excision repair proteins cooperate in the recognition of DNA interstrand crosslinks. Nucleic Acids Res 37:4420-9 (2009). WB ; Human . Read more (PubMed: 19468048) »

See 1 Publication for this product

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