概述

  • 产品名称
  • 描述
    兔多克隆抗体to MSH3
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from an internal region of human MSH3.

  • 阳性对照
    • Extracts from HUVEC cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
ELISA 1/20000.

靶标

  • 功能
    Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
  • 疾病相关
    Defects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
  • 序列相似性
    Belongs to the DNA mismatch repair mutS family. MSH3 subfamily.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Divergent upstream protein antibody
    • DNA mismatch repair protein antibody
    • DNA mismatch repair protein Msh 3 antibody
    • DNA mismatch repair protein MSH3 antibody
    • DUC 1 antibody
    • DUC1 antibody
    • DUG antibody
    • DUP antibody
    • hMSH3 antibody
    • MGC163306 antibody
    • MGC163308 antibody
    • Mismatch repair protein 1 antibody
    • MRP 1 antibody
    • MRP1 antibody
    • MSH 3 antibody
    • MSH3 antibody
    • MSH3_HUMAN antibody
    • MutS homolog 3 (E. coli) antibody
    • MutS homolog 3 antibody
    see all

Anti-MSH3 antibody 图像

  • All lanes : Anti-MSH3 antibody (ab69619) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 127 kDa
    Observed band size : 127 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

实验方案

Anti-MSH3 antibody (ab69619)参考文献

This product has been referenced in:
  • Zhao J  et al. Mismatch repair and nucleotide excision repair proteins cooperate in the recognition of DNA interstrand crosslinks. Nucleic Acids Res 37:4420-9 (2009). WB ; Human . Read more (PubMed: 19468048) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab69619.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册