Anti-MSH2抗体(Agarose) (ab1294)

概述

  • 产品名称Anti-MSH2抗体(Agarose)
    参阅全部 MSH2 一抗
  • 描述
    兔多克隆抗体to MSH2 (Agarose)
  • 偶联物Agarose
  • 经测试应用适用于: IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH - which represented aportion of human Homolog 2 of E. coli MutS encoded within exon 1 (LocusLink ID 4436).

  • 常规说明

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

性能

应用

Our Abpromise guarantee covers the use of ab1294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
  • 应用说明IP: Use at 15 to 25 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
    • 组织特异性Ubiquitously expressed.
    • 疾病相关Defects in MSH2 are the cause of hereditary non-polyposis colorectal cancer type 1 (HNPCC1) [MIM:120435]. Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term "suspected HNPCC" or "incomplete HNPCC" can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. MSH2 mutations may predispose to hematological malignancies and multiple cafe-au-lait spots.
      Defects in MSH2 are a cause of Muir-Torre syndrome (MuToS) [MIM:158320]; also abbreviated MTS. MuToS is a rare autosomal dominant disorder characterized by sebaceous neoplasms and visceral malignancy.
      Defects in MSH2 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
      Defects in MSH2 are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM.
    • 序列相似性Belongs to the DNA mismatch repair mutS family.
    • 翻译后修饰Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BAT26 antibody
      • COCA 1 antibody
      • COCA1 antibody
      • DNA mismatch repair protein Msh2 antibody
      • FCC 1 antibody
      • FCC1 antibody
      • hMSH2 antibody
      • HNPCC 1 antibody
      • HNPCC antibody
      • HNPCC1 antibody
      • LCFS2 antibody
      • MSH 2 antibody
      • Msh2 antibody
      • MSH2_HUMAN antibody
      • MutS homolog 2 antibody
      • MutS homolog 2 colon cancer nonpolyposis type 1 antibody
      • MutS protein homolog 2 antibody
      see all

    Anti-MSH2 antibody (Agarose) (ab1294)参考文献

    ab1294 has not yet been referenced specifically in any publications.

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    Thank you for contacting Abcam.

    All of our products are for research purposes only and are not certified for clinical research applications.

    I am sorry that I could not be more helpful in this matter.

    Thank you for your email. I understand that you are frustrated with these two antibodies. Abcam values all feedback from customers, both positive and negative. The quality of the products that we offer to our customers is very important to us, and beca...

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    The problem may be that the immobilized antibodies are not designed for regeneration. They are designed for a single time use with elution using SDS-PAGE sample buffer containing a reducing agent (e.g. BME). It is suggested that you should run a ge...

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    Thank you very much for your patience. I passed on your comments to the originator of ab1294 and ab1295. I agree that this is a problem, and appreciate your feedback regarding these antibodies. At this time I honestly don't know what is causing the pro...

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    Thank you for your patience. The originator of ab1294 and ab1295 was able to tell me that the specifications for the agarose beads used are - Agarose: 4% Exclusion limit: 20,000,000 Diameter: 60 to 160 um. If you have any more questions, please cont...

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    I am afraid the immunogen sequences are not available for these products so we do not have any further information on their similarity with mouse.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"