Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).
This product was changed from ascites to supernatant. Lot no’s high than GR128648-25 are from Tissue Culture Supernatant
Our Abpromise guarantee covers the use of ab52266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF | 1/200 - 1/1000. | |
IHC-Fr | 1/200 - 1/1000. | |
WB | 1/500 - 1/2000. Detects a band of approximately 98 kDa (predicted molecular weight: 105 kDa). | |
IHC-P | Use at an assay dependent concentration. | |
ELISA | 1/10000. | |
IP | Use a concentration of 5 µg/ml. |
ab52266 at 1/1000 dilution staining MSH2 in human Hela cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG1 was used as secondary antibody. The primary antibody shows green staining in image whilst actin filaments were stained red with Alexa Fluor® 555 phalloidin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"