- 数据表
- 具体的参考文献 (8)
- 实验方案
概述
- 产品名称Anti-MSH2抗体[3A2B8C]
参阅全部 MSH2 一抗 - 描述小鼠单克隆抗体[3A2B8C] to MSH2
- 经测试应用适用于: Flow Cyt, ICC/IF, IHC-Fr, WB, IHC-P, ELISA, IPmore details
- 种属反应性与反应: Human, Monkey
- 免疫原
Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).
- 阳性对照
- A431 cell lysate; human rectum carcinoma tissue.
- 常规说明
This product was changed from ascites to supernatant. Lot no’s high than GR128648-25 are from Tissue Culture Supernatant
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 纯度Tissue culture supernatant
- 克隆单克隆
- 克隆编号3A2B8C
- 同种型IgG1
- 研究领域
相关产品
- Compatible Secondaries
- Isotype control
- Related Products
应用
Our Abpromise guarantee covers the use of ab52266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. | |
| ICC/IF | 1/200 - 1/1000. | |
| IHC-Fr | 1/200 - 1/1000. | |
| WB | 1/500 - 1/2000. Detects a band of approximately 98 kDa (predicted molecular weight: 105 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ELISA | 1/10000. | |
| IP | Use a concentration of 5 µg/ml. |
靶标
- 功能Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
- 组织特异性Ubiquitously expressed.
- 疾病相关Defects in MSH2 are the cause of hereditary non-polyposis colorectal cancer type 1 (HNPCC1) [MIM:120435]. Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term "suspected HNPCC" or "incomplete HNPCC" can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. MSH2 mutations may predispose to hematological malignancies and multiple cafe-au-lait spots.
Defects in MSH2 are a cause of Muir-Torre syndrome (MuToS) [MIM:158320]; also abbreviated MTS. MuToS is a rare autosomal dominant disorder characterized by sebaceous neoplasms and visceral malignancy.
Defects in MSH2 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
Defects in MSH2 are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM. - 序列相似性Belongs to the DNA mismatch repair mutS family.
- 翻译后修饰Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
Phosphorylated upon DNA damage, probably by ATM or ATR. - 细胞定位Nucleus.
- Information by UniProt
-
数据库链接
- Entrez Gene: 4436 Human
- Omim: 609309 Human
- SwissProt: P43246 Human
- Unigene: 597656 Human
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别名
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Anti-MSH2 antibody [3A2B8C] 图像
-
![Immunocytochemistry/ Immunofluorescence - MSH2 antibody [3A2B8C] (ab52266)](http://www.abcam.cn/ps/datasheet/Images/52/ab52266/ab52266_rev9156_2.jpg)
Immunocytochemistry/ Immunofluorescence - MSH2 antibody [3A2B8C] (ab52266)This image is courtesy of an Abreview submitted by Dr Kirk McManusab52266 (1/200) detecting MSH2 in HeLa cells (green). Cells were fixed in methanol (-20'C, 10min) and counterstained with DAPI in order to highlight the nucleus. Please refer to abreview for further experimental details. -
![Western blot - MSH2 antibody [3A2B8C] (ab52266)](http://www.abcam.cn/ps/datasheet/images/52/ab52266/MSH2-Primary-antibodies-ab52266-2.jpg)
Western blot - MSH2 antibody [3A2B8C] (ab52266)All lanes : Anti-MSH2 antibody [3A2B8C] (ab52266) at 1/2000 dilution
Lane 1 : Cell lysates prepared from human Hela cells
Lane 2 : Cell lysates prepared from A549 cells
Lane 3 : Cell lysates prepared from human A431 cells
Lane 4 : Cell lysates prepared from HEK293 cells
Lysates/proteins at 100 µg per lane.
Secondary
HRP-conjugated Goat polyclonal to mouse IgG
Predicted band size : 105 kDa -
![Immunocytochemistry/ Immunofluorescence - MSH2 antibody [3A2B8C] (ab52266)](http://www.abcam.cn/ps/datasheet/images/52/ab52266/MSH2-Primary-antibodies-ab52266-3.jpg)
Immunocytochemistry/ Immunofluorescence - MSH2 antibody [3A2B8C] (ab52266)ab52266 at 1/1000 dilution staining MSH2 in human Hela cells by Immunocytochemistry/ Immunofluorescence. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG1 was used as secondary antibody. The primary antibody shows green staining in image whilst actin filaments were stained red with Alexa Fluor® 555 phalloidin.
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](http://www.abcam.cn/ps/datasheet/images/52/ab52266/MSH2-Primary-antibodies-ab52266-4.jpg)
Flow Cytometry-Anti-MSH2 antibody [3A2B8C](ab52266)Overlay histogram showing HeLa cells stained with ab52266 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52266, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunoprecipitation - Anti-MSH2 antibody [3A2B8C] (ab52266)](http://www.abcam.cn/ps/datasheet/images/52/ab52266/MSH2-Primary-antibodies-ab52266-5.jpg)
Immunoprecipitation - Anti-MSH2 antibody [3A2B8C] (ab52266)MSH2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Mouse monoclonal to MSH2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52266.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/10,000 dilution.
Band: 105kDa; MSH2 -
![Immunohistochemistry (Paraffin-embedded sections) - MSH2 antibody [3A2B8C] (ab52266)](http://www.abcam.cn/ps/datasheet/Images/52/ab52266/ab52266ihc.jpg)
Immunohistochemistry (Paraffin-embedded sections) - MSH2 antibody [3A2B8C] (ab52266)Immunohistochemical analysis of paraffin-embedded human rectum carcinoma tissue, showing nuclear and cytoplasmic localisation, using ab52266 at a dilution of 1/200 - 1/1000 with DAB staining.
Anti-MSH2 antibody [3A2B8C] (ab52266)参考文献
This product has been referenced in:
- Cinesi C et al. Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase. Nat Commun 7:13272 (2016). WB . Read more (PubMed: 27827362) »
- David R et al. Synergistic and Antagonistic Mutation Responses of Human MCL-5 Cells to Mixtures of Benzo[a]pyrene and 2-Amino-1-Methyl-6-Phenylimidazo[4,5-b]pyridine: Dose-Related Variation in the Joint Effects of Common Dietary Carcinogens. Environ Health Perspect 124:88-96 (2016). Read more (PubMed: 26091049) »
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