概述

  • 产品名称Anti-MSF抗体
    参阅全部 MSF 一抗
  • 描述
    兔多克隆抗体to MSF
  • 经测试应用适用于: IP, IHC-Pmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Rhesus monkey
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 536 - 586 of Human MSF (NP_001106963.1).

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH: 7 to 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab114099 was affinity purified using an epitope specific to MSF immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab114099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.
    • 组织特异性Widely expressed. Isoforms are differentially expressed in testes, kidney, liver heart, spleen, brain, peripheral blood leukocytes, skeletal muscle and kidney. Specific isoforms appear to demonstrate tissue specificity. Isoform 5 is the most highly expressed in fetal tissue. Isoform 1 is detected in all tissues except the brain and thymus, while isoform 2, isoform 3, and isoform 4 are detected at low levels in approximately half of the fetal tissues.
    • 疾病相关Note=A chromosomal aberration involving SEPT9/MSF is found in therapy-related acute myeloid leukemia (t-AML). Translocation t(11;17)(q23;q25) with MLL.
      Defects in SEPT9 are a cause of hereditary neuralgic amyotrophy (HNA) [MIM:162100]; also known as neuritis with brachial predilection (NAPB) or hereditary brachial plexus neuropathy or hereditary neuralgic amyotrophy with predilection for brachial plexus. HNA is an autosomal dominant form of recurrent focal neuropathy characterized clinically by acute, recurrent episodes of brachial plexus neuropathy with muscle weakness and atrophy preceded by severe pain in the affected arm. HNA is triggered by environmental factors such as infection or parturition.
    • 序列相似性Belongs to the septin family.
    • 细胞定位Cytoplasm > cytoskeleton. In an epithelial cell line, concentrates at cell-cell contact areas. After TGF-beta1 treatment and induction of epithelial to mesenchymal transition, colocalizes partly with actin stress fibers. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AF17q25 antibody
      • Cell division control protein septin D1 antibody
      • KIAA0991 antibody
      • MLL septin like fusion protein antibody
      • MLL septin-like fusion protein antibody
      • MLL septin-like fusion protein MSF-A antibody
      • MSF antibody
      • MSF1 antibody
      • NAPB antibody
      • Ov/Br septin antibody
      • Ovarian/breast septin alpha antibody
      • Ovarian/Breast septin antibody
      • PNUTL4 antibody
      • SEPT9 antibody
      • SEPT9_HUMAN antibody
      • SeptD1 antibody
      • Septin 9 antibody
      • Septin D1 antibody
      • Septin-9 antibody
      • SINT1 antibody
      see all

    Anti-MSF antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MSF with ab114099 at 1/1000 (1µg/ml). A DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.
    • Rabbit anti-MSF antibody, at 1 µg/ml, staining MSF in HeLa whole cell lysate immunoprecipitated using ab114099 at 3 µg/mg lysate (1 mg for IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 1 second.

    Anti-MSF antibody (ab114099)参考文献

    ab114099 has not yet been referenced specifically in any publications.

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