概述

  • 产品名称Anti-MRPS21抗体
  • 描述
    兔多克隆抗体to MRPS21
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    synthetic peptide derived from internal of human MRPS21.

  • 阳性对照
    • human pancreas tissue, extracts from COS cells, MCF-7 cells

性能

应用

Our Abpromise guarantee covers the use of ab74101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 11 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

  • 相关性 Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S21P family. Pseudogenes corresponding to this gene are found on chromosomes 1p, 1q, 9p, 10p, 10q, 16q, and 17q. Available sequence data analyses identified splice variants that differ in the 5' UTR; both transcripts encode the same protein. [provided by RefSeq]
  • 细胞定位Mitochondrial small ribosomal subunit
  • 数据库链接
  • 别名
    • MDS016 antibody
    • mitochondrial 28S ribosomal protein S21 antibody
    • Mitochondrial ribosomal protein S21 antibody
    • MRP S21 antibody
    • S21mt antibody
    see all

Anti-MRPS21 antibody 图像

  • All lanes : Anti-MRPS21 antibody (ab74101) at 1/500 dilution

    Lane 1 : extracts from COS cells
    Lane 2 : extracts from COS cells with immunizing peptide at 5 µg

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 11 kDa
    Observed band size : 11 kDa
  • Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using 1/50 ab74101 with (right panel) or without (left panel) preincubation with immunizing peptide.
  • Immunofluorescence analysis of MCF-7 cells, using 1/500 ab74101 with (right panel) or without (left panel) preincubation with immunizing peptide.

Anti-MRPS21 antibody (ab74101)参考文献

ab74101 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"