概述

  • 产品名称
  • 描述
    山羊多克隆抗体to MRPL3
  • 经测试应用
    适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    NVCQPGAPSITFA

    , corresponding to C terminal amino acids 336-348 of Human MRPL3

  • 阳性对照
    • This antibody gave a positive result when used in the following methanol fixed cell lines: Caco-2

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab39268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration. PubMed: 20144953

This antibody gave a positive result in ELISA against the immunizing peptide. Antibody detection limit dilution >1:32,000.

WB Use at an assay dependent concentration.

靶标

  • 序列相似性
    Belongs to the ribosomal protein L3P family.
  • 细胞定位
    Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 39S ribosomal protein L3 antibody
    • 39S ribosomal protein L3 mitochondrial antibody
    • COXPD9 antibody
    • L3mt antibody
    • Mitochondrial 39S ribosomal protein L3 antibody
    • Mitochondrial 60S ribosomal protein L3 antibody
    • mitochondrial antibody
    • mitochondrial ribosomal protein antibody
    • Mitochondrial ribosomal protein L3 antibody
    • MRL3 antibody
    • MRP-L3 antibody
    • MRPL3 antibody
    • RM03_HUMAN antibody
    • RPML3 antibody
    see all

图片

  • ab39268 stained Caco-2 cells. The cells were 100% methamol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39268 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was 150133 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

文献

This product has been referenced in:
  • Zaganelli S  et al. The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules. J Biol Chem 292:4519-4532 (2017). Read more (PubMed: 28082677) »
  • Rozanska A  et al. The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome. Biochem J 474:2145-2158 (2017). WB . Read more (PubMed: 28512204) »

See all 10 Publications for this product

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