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Recombinant MRP8/14 heterocomplex (Human). The antigen is produced by the Ca2+ dependent heterocomplex formation of MRP8 (S100A8 or Calgranulin A) and MRP14 (S100A9 or Calgranulin B), two calcium binding proteins of the S 100 protein family.
In immune histochemistry, this antibody detects early inflammatory macrophages and can thus be used for the classification of acute stage inflammation in tissue sections and in smears.
Our Abpromise guarantee covers the use of ab51863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 1 µg/ml.
|IHC-P||Use a concentration of 4 µg/ml.
1/250. Proteinase K pretreatment for antigen retrieval is recommended.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"