概述

  • 产品名称
    Anti-MRP8 + MRP14抗体(Biotin)
    参阅全部 MRP8 + MRP14 一抗
  • 描述
    鸡多克隆抗体to MRP8 + MRP14 (Biotin)
  • 偶联物
    Biotin
  • 特异性
    Does not discriminate between the individual subunits MRP8 or MRP14.
  • 经测试应用
    适用于: ELISA, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant MRP8/14 heterocomplex (Human). The antigen is produced by the Ca2+ dependent heterocomplex formation of MRP8 (S100A8 or Calgranulin A) and MRP14 (S100A9 or Calgranulin B), two calcium binding proteins of the S 100 protein family.

  • 阳性对照
    • Human tonsil.
  • 常规说明
    Peripheral blood monocytes carry the antigen extra and intracellularly, neutrophils only intracellularly. The antigen is present on macrophages in the red pulp of the spleen and in the liver. It is also strongly expressed on macrophages in acute inflamed tissues (peridontitis, contact excema, urticaria, erythrodermia).


    In immune histochemistry, this antibody detects early inflammatory macrophages and can thus be used for the classification of acute stage inflammation in tissue sections and in smears.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: Kathon
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    IgY fraction
  • 纯化说明
    Purified from egg yolk by repeated precipitation.
  • Primary antibody说明
    In immune histochemistry, this antibody detects early inflammatory macrophages and can thus be used for the classification of acute stage inflammation in tissue sections and in smears.
  • 克隆
    多克隆
  • 同种型
    IgY
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IHC-Fr Use a concentration of 1 µg/ml.

1/1000.

IHC-P Use a concentration of 4 µg/ml.

1/250. Proteinase K pretreatment for antigen retrieval is recommended.

靶标

  • 相关性
    MRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
  • 细胞定位
    Cytoplasmic and Nuclear
  • 数据库链接
  • 别名
    • CAGA antibody
    • CAGB antibody
    • Calgranulin A antibody
    • Calgranulin B antibody
    • Calprotectin L1L subunit antibody
    • CFAG antibody
    • CP10 antibody
    • Cystic fibrosis antigen antibody
    • Leukocyte L1 complex light chain antibody
    • Macrophage L1 protein Calprotectin antibody
    • Migration inhibitory factor related protein 14 antibody
    • Migration inhibitory factor related protein 8 antibody
    • MRP-14 antibody
    • MRP-8 antibody
    • P14 antibody
    • P8 antibody
    • S100 calcium binding protein A8 antibody
    • S100 calcium binding protein A9 antibody
    • S100A8 antibody
    • S100A9 antibody
    • Urinary stone protein band A antibody
    see all

图片

  • ab51863 at 4µg/ml staining human spleen; paraffin embedded.

文献

This product has been referenced in:
  • Lood C  et al. Protein synthesis of the pro-inflammatory S100A8/A9 complex in plasmacytoid dendritic cells and cell surface S100A8/A9 on leukocyte subpopulations in systemic lupus erythematosus. Arthritis Res Ther 13:R60 (2011). ELISA . Read more (PubMed: 21492422) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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