Anti-MRP8 + MRP14抗体[27E10] (ab17050)

概述

  • 产品名称
    Anti-MRP8 + MRP14抗体[27E10]
    参阅全部 MRP8 + MRP14 一抗
  • 描述
    小鼠单克隆抗体[27E10] to MRP8 + MRP14
  • 特异性
    Does not react with lymphocytes or platelets.
  • 经测试应用
    适用于: ICC/IF, WB, IP, Flow Cyt, IHC-Fr, IHC-Pmore details
    不适用于: FuncS
  • 种属反应性
    与反应: Human, Rhesus monkey
    不与反应: Mouse
  • 免疫原

    Human blood monocytes.

  • 表位
    ab17050 recognises an epitope on the MRP8 + MRP14 complex which is not exposed on the individual subunits.
  • 阳性对照
    • Human granulocytes
  • 常规说明

    ab17050 can be used for early detection of inflammatory macrophages, characterisation of tumorous tissues and monitoring of peripheral blood cell cultures.

性能

应用

Our Abpromise guarantee covers the use of ab17050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/10. Perform under non-denaturing conditions only.
IP Use at an assay dependent concentration.
Flow Cyt 1/10. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/10.
IHC-P Use at an assay dependent concentration. PubMed: 21517798
  • 应用说明
    Is unsuitable for FuncS.
  • 靶标

    • 相关性
      MRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
    • 细胞定位
      Cytoplasmic and Nuclear
    • 数据库链接
    • 别名
      • CAGA antibody
      • CAGB antibody
      • Calgranulin A antibody
      • Calgranulin B antibody
      • Calprotectin L1L subunit antibody
      • CFAG antibody
      • CP10 antibody
      • Cystic fibrosis antigen antibody
      • Leukocyte L1 complex light chain antibody
      • Macrophage L1 protein Calprotectin antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor related protein 8 antibody
      • MRP-14 antibody
      • MRP-8 antibody
      • P14 antibody
      • P8 antibody
      • S100 calcium binding protein A8 antibody
      • S100 calcium binding protein A9 antibody
      • S100A8 antibody
      • S100A9 antibody
      • Urinary stone protein band A antibody
      see all

    文献

    This product has been referenced in:
    • Val S  et al. Proteomic Characterization of Middle Ear Fluid Confirms Neutrophil Extracellular Traps as a Predominant Innate Immune Response in Chronic Otitis Media. PLoS One 11:e0152865 (2016). IF . Read more (PubMed: 27078692) »
    • Carrión M  et al. IL-22/IL-22R1 axis and S100A8/A9 alarmins in human osteoarthritic and rheumatoid arthritis synovial fibroblasts. Rheumatology (Oxford) 52:2177-86 (2013). Human . Read more (PubMed: 24056519) »

    See all 8 Publications for this product

    客户评价及客户问答

    Thank you for your enquiry.

    I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any MRP8 and MRP14 antibody tested ...

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    Thank you for contacting us.

    The supplying lab send me the following information:

    Unfortunately we do not have a detailled protocol for use on paraffin sections. The application has been added in the past upon personal communicati...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Oral Dysplastic Epithelium cells)
    Specification
    Oral Dysplastic Epithelium cells
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Enzymatic
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 2%
    Username

    Mr. Cancer center

    Verified customer

    提交于 Jul 25 2006

    For the generation of the antibody human blood monocytes are used. We regret to inform you that we have no more information about the immunogen. Furthermore we have no information about the reactivity with rat Calprotectin. Should you decide to go ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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