Anti-MRP8抗体(ab75519)

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概述

  • 产品名称
    Anti-MRP8抗体
    参阅全部 MRP8 一抗
  • 描述
    兔多克隆抗体to MRP8
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Human MRP8.

    参阅Abcam的专有抗源政策

    (Peptide available as ab100827.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human Tissue Lysates: Spleen; Bone Marrow

性能

应用

Our Abpromise guarantee covers the use of ab75519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

靶标

  • 功能
    Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
  • 组织特异性
    Expressed by macrophages in chronic inflammations. Also expressed in epithelial cells constitutively or induced during dermatoses.
  • 序列相似性
    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 细胞定位
    Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
  • Information by UniProt
  • 数据库链接
    • 别名
      • 60B8Ag antibody
      • AI323541 antibody
      • B8Ag antibody
      • BEE11 antibody
      • CAGA antibody
      • Calgranulin-A antibody
      • Calprotectin L1L subunit antibody
      • Calprotectin, included antibody
      • CFAG antibody
      • CGLA antibody
      • Chemotactic cytokine CP-10 antibody
      • CP-10 antibody
      • Cystic fibrosis antigen antibody
      • L1Ag antibody
      • Leukocyte L1 complex light chain antibody
      • MA387 antibody
      • MIF antibody
      • Migration inhibitory factor-related protein 8 antibody
      • MRP-8 antibody
      • Myeloid-related protein 8 antibody
      • Neutrophil cytosolic 7 kDa protein antibody
      • NIF antibody
      • p8 antibody
      • Pro-inflammatory S100 cytokine antibody
      • Protein S100-A8 antibody
      • S100 calcium binding protein A8 (calgranulin A) antibody
      • S100 calcium binding protein A8 antibody
      • S100 calcium-binding protein A8 antibody
      • S100A8 antibody
      • S100A8/S100A9 complex, included antibody
      • S10A8_HUMAN antibody
      • Urinary stone protein band A antibody
      see all

    图片

    • Western blot - Anti-MRP8 antibody (ab75519)
      Western blot - Anti-MRP8 antibody (ab75519)
      All lanes : Anti-MRP8 antibody (ab75519) at 1 µg/ml

      Lane 1 : Human spleen tissue lysate - total protein (ab29699)
      Lane 2 : Bone Marrow (Human) Tissue Lysate - adult normal tissue

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 11 kDa
      Observed band size: 11 kDa


      Exposure time: 20 minutes
    • Immunocytochemistry/ Immunofluorescence - Anti-MRP8 antibody (ab75519)
      Immunocytochemistry/ Immunofluorescence - Anti-MRP8 antibody (ab75519)

      ICC/IF image of ab75519 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab75519 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    文献

    ab75519 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab75519？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    Looking for dog specific anti S100A8 and S100A9 antibodies.

    I have checked the immunogen compatibility of all MRP8 antibodies - 2 antibodies ab75519 and ab126721 has homology more than 70% with human protein so these antibodies will be worth trying. I am happy to offer you Abtrial codes for these. Please check ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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