概述
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产品名称Anti-MRP8抗体
参阅全部 MRP8 一抗 -
描述兔多克隆抗体to MRP8
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经测试应用适用于: ICC/IF, WBmore details
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种属反应性与反应: Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Human MRP8.
(Peptide available as ab100827.)
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阳性对照
- This antibody gave a positive signal in the following Human Tissue Lysates: Spleen; Bone Marrow
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab75519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
靶标
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功能Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
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组织特异性Expressed by macrophages in chronic inflammations. Also expressed in epithelial cells constitutively or induced during dermatoses.
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序列相似性Belongs to the S-100 family.
Contains 2 EF-hand domains. -
细胞定位Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
- Information by UniProt
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数据库链接
- Entrez Gene: 6279 Human
- Omim: 123885 Human
- SwissProt: P05109 Human
- Unigene: 416073 Human
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别名
- 60B8Ag antibody
- AI323541 antibody
- B8Ag antibody
see all
Anti-MRP8 antibody 图像
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Western blot - MRP8 antibody (ab75519)All lanes : Anti-MRP8 antibody (ab75519) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 11 kDa
Observed band size : 11 kDa
Exposure time : 20 minutes -
Immunocytochemistry/ Immunofluorescence - MRP8 antibody (ab75519)ICC/IF image of ab75519 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab75519 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-MRP8 antibody (ab75519)参考文献
ab75519 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"