Anti-MRP8抗体[2C5/4] (ab19860)

概述

  • 产品名称Anti-MRP8抗体[2C5/4]
    参阅全部 MRP8 一抗
  • 描述
    小鼠单克隆抗体[2C5/4] to MRP8
  • 经测试应用适用于: Flow Cyt, IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human MRP8

  • 阳性对照
    • Positive control: neutrophil lysate (particularly neutrophils from spleen)

性能

应用

Our Abpromise guarantee covers the use of ab19860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 8 kDa.

靶标

  • 功能Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
  • 组织特异性Expressed by macrophages in chronic inflammations. Also expressed in epithelial cells constitutively or induced during dermatoses.
  • 序列相似性Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 细胞定位Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 60B8Ag antibody
    • AI323541 antibody
    • B8Ag antibody
    • BEE11 antibody
    • CAGA antibody
    • Calgranulin-A antibody
    • Calprotectin L1L subunit antibody
    • Calprotectin, included antibody
    • CFAG antibody
    • CGLA antibody
    • Chemotactic cytokine CP-10 antibody
    • CP-10 antibody
    • Cystic fibrosis antigen antibody
    • L1Ag antibody
    • Leukocyte L1 complex light chain antibody
    • MA387 antibody
    • MIF antibody
    • Migration inhibitory factor-related protein 8 antibody
    • MRP-8 antibody
    • Myeloid-related protein 8 antibody
    • Neutrophil cytosolic 7 kDa protein antibody
    • NIF antibody
    • p8 antibody
    • Pro-inflammatory S100 cytokine antibody
    • Protein S100-A8 antibody
    • S100 calcium binding protein A8 (calgranulin A) antibody
    • S100 calcium binding protein A8 antibody
    • S100 calcium-binding protein A8 antibody
    • S100A8 antibody
    • S100A8/S100A9 complex, included antibody
    • S10A8_HUMAN antibody
    • Urinary stone protein band A antibody
    see all

Anti-MRP8 antibody [2C5/4] 图像

  • Overlay histogram showing THP1 cells stained with ab19860 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19860, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • IHC image of ab19860 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab19860, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-MRP8 antibody [2C5/4] (ab19860)参考文献

ab19860 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your phone enquiry. I apologise for the long delay in responding to your query. Unfortunately we have no experimental data available on the cross reactivity of this MRP-8 antibody (ab19860) with MRP-14. However, I can confirm that the ...

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