概述

  • 产品名称
  • 描述
    兔多克隆抗体to MRP4
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MRP4.

    (Peptide available as ab37589.)

  • 阳性对照
    • ab32550 gave a positive signal in the following Human Tissue Lysates: Testis, Spleen, Thymus, Kidney and Prostate.

性能

应用

Our Abpromise guarantee covers the use of ab32550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 230 kDa (predicted molecular weight: 150 kDa).

靶标

  • 功能
    May be an organic anion pump relevant to cellular detoxification.
  • 组织特异性
    Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.
  • 序列相似性
    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABCC 4 antibody
    • ABCC4 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 4 antibody
    • ATP binding cassette sub family C member 4 antibody
    • ATP-binding cassette sub-family C member 4 antibody
    • bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4) antibody
    • bA464I2.1 antibody
    • Canalicular multispecific organic anion transporter antibody
    • Canalicular multispecific organic anion transporter ABC superfamily antibody
    • EST170205 antibody
    • MOAT B antibody
    • MOAT-B antibody
    • MOATB antibody
    • MRP 4 antibody
    • MRP/cMOAT related ABC transporter antibody
    • MRP/cMOAT-related ABC transporter antibody
    • MRP4_HUMAN antibody
    • Multi specific organic anion transporter B antibody
    • Multi-specific organic anion transporter B antibody
    • Multidrug resistance associated protein 4 antibody
    • Multidrug resistance-associated protein 4 antibody
    • OTTHUMP00000018560 antibody
    see all

图片

  • All lanes : Anti-MRP4 antibody (ab32550) at 1 µg/ml

    Lane 1 : Prostate (Human) Tissue Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 150 kDa
    Observed band size: 230 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 32 kDa, 42 kDa, 53 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    MRP4 has a predicted molecular weight of 150 kDa; however it has a number of potential transmembrane regions, glycosylation and phosphotylation sites which may affect the migration of the protein (SwissProt data). Abcam currently sells another antibody (ab56675) which detects a band of similar molecular weight in Hela lysate.

实验方案

文献

This product has been referenced in:
  • Dolezelova E  et al. Resveratrol modifies biliary secretion of cholephilic compounds in sham-operated and cholestatic rats. World J Gastroenterol 23:7678-7692 (2017). Read more (PubMed: 29209109) »
  • Brcakova E  et al. Alteration of methotrexate biliary and renal elimination during extrahepatic and intrahepatic cholestasis in rats. Biol Pharm Bull 32:1978-85 (2009). WB ; Rat . Read more (PubMed: 19952415) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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