MRP4 has a predicted molecular weight of 150 kDa; however it has a number of potential transmembrane regions, glycosylation and phosphotylation sites which may affect the migration of the protein (SwissProt data). Abcam currently sells another antibody (ab56675) which detects a band of similar molecular weight in Hela lysate.
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Brcakova E et al. Alteration of methotrexate biliary and renal elimination during extrahepatic and intrahepatic cholestasis in rats. Biol Pharm Bull32:1978-85 (2009).
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