The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
ICC: Use at 1/20-1/50 dilution on acetone fixed cytospin preparations.
IHC-Fr: Use at 1/20 dilution. Acetone fixed.
WB: 1/20-1/50. Detects a band of approximately 190-200 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.