Anti-MRP1抗体[MRPm5] (ab24102)


  • 产品名称Anti-MRP1抗体[MRPm5]
    参阅全部 MRP1 一抗
  • 描述
    小鼠单克隆抗体[MRPm5] to MRP1
  • 特异性MRPm5 does not cross-react with the human MDR1 and MDR3 gene products.
  • 经测试应用适用于: IHC-Fr, IHC-P, Flow Cyt, WB, ICCmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Fusion protein, corresponding to amino acids 986-1204 of Human MRP1.

  • 表位MRP1 antibody reacts with an internal epitope of MRP1.



Our Abpromise guarantee covers the use of ab24102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/20.
IHC-P 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval in citrate buffer (pH 6.0) for 10 minutes before commencing with IHC staining protocol.

Flow Cyt 1/20 - 1/50. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB 1/20 - 1/50. Predicted molecular weight: 190 kDa.
ICC 1/20 - 1/50.


  • 功能Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
  • 组织特异性Lung, testis and peripheral blood mononuclear cells.
  • 序列相似性Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABC 29 antibody
    • ABC29 antibody
    • ABCC 1 antibody
    • ABCC antibody
    • Abcc1 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 1 antibody
    • ATP binding cassette sub-family C member 1 antibody
    • ATP-binding cassette sub-family C member 1 antibody
    • DKFZp686N04233 antibody
    • DKFZp781G125 antibody
    • GS X antibody
    • GSX antibody
    • Leukotriene C(4) transporter antibody
    • LTC4 transporter antibody
    • MRP 1 antibody
    • MRP antibody
    • MRP1_HUMAN antibody
    • Multidrug resistance associated protein 1 antibody
    • Multidrug resistance protein antibody
    • Multidrug resistance-associated protein 1 antibody
    • Multiple drug resistance associated protein antibody
    • Multiple drug resistance protein 1 antibody
    see all

Anti-MRP1 antibody [MRPm5] 图像

  • ICC/IF image of Anti-MRP1 antibody (ab24102) stained human ES cell derived hepatocytes. The cells were fixed in methanol, blocked and permeabilized using 10% BSA in PBS/0.1% Tween, for 1 hour at 20oC. The cells were then incubated with ab24102 at a 1/50 dilution for 16 hours at 4oC. The secondary antibody was a Donkey anti-Mouse Alexa Fluor 488, used at a 1/400 dilution.

    See Abreview

  • Overlay histogram showing HL60 cells stained with ab24102 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24102, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-MRP1 antibody [MRPm5] (ab24102)参考文献

This product has been referenced in:
  • Ji L  et al. Correlation of Nrf2, NQO1, MRP1, cmyc and p53 in colorectal cancer and their relationships to clinicopathologic features and survival. Int J Clin Exp Pathol 7:1124-31 (2014). IHC-P ; Human . Read more (PubMed: 24695690) »
  • Helms HC  et al. An electrically tight in vitro blood-brain barrier model displays net brain-to-blood efflux of substrates for the ABC transporters, P-gp, Bcrp and Mrp-1. AAPS J 16:1046-55 (2014). Read more (PubMed: 24934296) »

See all 7 Publications for this product

Product Wall

I'm assuming you're referring to a lysis buffer for sample preparation prior to western blot. If this is the case, since MRP1 is a membrane bound protein we recommend using RIPA buffer for lysis. Our 10X RIPA buffer ab156034 is through the link below:<...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Human Cell (ES - derived hepatocytes)
Specification ES - derived hepatocytes
Permeabilization Yes - During blocking step, BSA is diluted in PBS/0.1% Tween.
Fixative Methanol

Abcam user community

Verified customer

提交于 Aug 23 2013

Thank you for contacting us.

I do hope the suggestions made today shed some light upon the issue you are experiencing with ab24102 Anti-MRP1 antibody [MRPm5] and look forward to hear the results.

This product is covered by our Abpro...

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Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab241...

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Thank you for your enquiry and your interest in our products.

Here are a few antibodies against the following targets which have been tested and characterized for Flow cytometry. Please take a look at the on-line datasheets for further inform...

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Thank you for contacting us. We do have products that are conjugated to match your needs, Ab81424 Anti-CD44 antibody [MEM-263] is conjugated with APC. Ab58754 Anti-CD44 antibody [MEM-263] (Phycoerythrin). I've included links to each below: http://www.a...

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Thank you for contacting Abcam. We offer a number of PGP antibodies that are guaranteed to work in Flow Cytometry on dog cells. I would recommend the FITC conjugated

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Thank you for contacting us. ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are ab3083; ab3366; ab10333; ab80594; ab80626. Please check the da...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Liver)
Gel Running Conditions Reduced Denaturing (Samples were not denatured prior to gel loading)
Loading amount 80 µg
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

Abcam user community

Verified customer

提交于 Feb 14 2011

The only recommendation I have seen for a western blot positive control is a lysate from cells transfected with MRP1. We do not have such a lysate or a recombinant protein. As I mentioned in our phone call, endogenous sources include lung, testis, and ...

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