Anti-MRP1抗体[IU2H10] (ab32574)

概述

  • 产品名称
    Anti-MRP1抗体[IU2H10]
    参阅全部 MRP1 一抗
  • 描述
    小鼠单克隆抗体[IU2H10] to MRP1
  • 宿主
    Mouse
  • 特异性
    This antibody is specific for MRP1 protein.
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment: MALRGFCSAD GSDPLWDWNV TWNTSNPDFT KCF, corresponding to amino acids 1-33 of Human MRP1

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab24102 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab32574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Predicted molecular weight: 172 kDa. There may be a lower MW band that is a degradation product.
ICC/IF Use at an assay dependent dilution.

靶标

  • 功能
    Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
  • 组织特异性
    Lung, testis and peripheral blood mononuclear cells.
  • 序列相似性
    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ABC 29 antibody
    • ABC29 antibody
    • ABCC 1 antibody
    • ABCC antibody
    • Abcc1 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 1 antibody
    • ATP binding cassette sub-family C member 1 antibody
    • ATP binding cassette subfamily C member 1 antibody
    • ATP binding cassette transporter variant ABCC1delta ex13 antibody
    • ATP binding cassette transporter variant ABCC1delta ex13&14 antibody
    • ATP binding cassette transporter variant ABCC1delta ex25 antibody
    • ATP binding cassette transporter variant ABCC1delta ex25&26 antibody
    • ATP binding cassette, sub-family C (CFTR/MRP), member 1 antibody
    • ATP-binding cassette sub-family C member 1 antibody
    • DKFZp686N04233 antibody
    • DKFZp781G125 antibody
    • GS X antibody
    • GSX antibody
    • Leukotriene C(4) transporter antibody
    • LTC4 transporter antibody
    • MRP 1 antibody
    • MRP antibody
    • MRP1 antibody
    • MRP1_HUMAN antibody
    • Multidrug resistance associated protein 1 antibody
    • Multidrug resistance protein antibody
    • Multidrug resistance-associated protein 1 antibody
    • Multiple drug resistance associated protein antibody
    • Multiple drug resistance protein 1 antibody
    see all

图片

  • All lanes : Anti-MRP1 antibody [IU2H10] (ab32574)

    Lane 1 : Vector-transfected HEK293 cells
    Lane 2 : MRP1-transfected cells, prep1
    Lane 3 : MRP1-transfected cells, prep2 (the lower band is a degradation product)

    Predicted band size: 172 kDa

  • Ab32574 staining MRP1 in HeLa cells by Immunocytochemistry/Immunofluorescence (ICC/IF). Samples were incubated with primary antibody at 1:50 dilution against Dylight 488 (green). Alpha tubulin and nuclei were counterstained with Dylight 550 (red) and DAPI (blue) respectively.

文献

This product has been referenced in:
  • You Y  et al. Zoledronic acid reverses cisplatin resistance in nasopharyngeal carcinoma cells by activating the mitochondrial apoptotic pathway. Oncol Lett 13:1840-1846 (2017). WB ; Human . Read more (PubMed: 28454332) »
  • Zhang C  et al. Growth of tyrosine kinase inhibitor-resistant Philadelphia-positive acute lymphoblastic leukemia: Role of bone marrow stromal cells. Oncol Lett 13:2059-2070 (2017). WB . Read more (PubMed: 28454362) »

See all 16 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Cell lysate - whole cell (brain endothelial cells)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
35 µg
Specification
brain endothelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Dec 19 2017

Thank you for contacting us.

For ab108312, you could use human fetal liver (ab29890), JAR, 293T (ab95494), HepG2 (ab7900) or human placenta (ab29745) lysates as a positive control.

For ab32574, you could consider using MRP-1 trans...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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