重组Anti-MRP1抗体[EPR4658(2)] - C-terminal (ab180960)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4658(2)] to MRP1 - C-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MRP1抗体[EPR4658(2)] - C-terminal
参阅全部 MRP1 一抗 -
描述
兔单克隆抗体[EPR4658(2)] to MRP1 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- A431: HeLa and A549 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR4658(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180960于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 171 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 171 kDa. |
靶标
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功能
Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency. -
组织特异性
Lung, testis and peripheral blood mononuclear cells. -
序列相似性
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4363 Human
- Omim: 158343 Human
- SwissProt: P33527 Human
- Unigene: 391464 Human
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别名
- ABC 29 antibody
- ABC29 antibody
- ABCC 1 antibody
see all
图片
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All lanes : Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/1000 dilution
Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : ABCC1 knockout A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 171 kDaLanes 1 - 2: Merged signal (red and green). Green - ab180960 observed at 170 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab180960 was shown to specifically react with ABCC1 in wild-type A549 cells as signal was lost in ABCC1 knockout cells. Wild-type and ABCC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab180960 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging
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All lanes : Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate (boiled)
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 171 kDa
Exposure time: 20 secondsBlocking and dilution buffer: 5% NFDM/TBST.
If there is no ideal result on boiled sample, we suggest not to boil sample before loading onto the gel.
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Anti-MRP1 antibody [EPR4658(2)] - C-terminal (ab180960) at 1/5000 dilution + A549 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 171 kDa
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (11)
ab180960 被引用在 11 文献中.
- Zeng W et al. Elevated N-Glycosylation Contributes to the Cisplatin Resistance of Non-Small Cell Lung Cancer Cells Revealed by Membrane Proteomic and Glycoproteomic Analysis. Front Pharmacol 12:805499 (2021). PubMed: 35002739
- Low FG et al. Roles of ABCC1 and ABCC4 in Proliferation and Migration of Breast Cancer Cell Lines. Int J Mol Sci 21:N/A (2020). PubMed: 33081264
- Wang C et al. Silencing long non-coding RNA XIST suppresses drug resistance in acute myeloid leukemia through down-regulation of MYC by elevating microRNA-29a expression. Mol Med 26:114 (2020). PubMed: 33228517
- Li Y et al. Involvement of the PI3K/Akt/Nrf2 Signaling Pathway in Resveratrol-Mediated Reversal of Drug Resistance in HL-60/ADR Cells. Nutr Cancer 71:1007-1018 (2019). PubMed: 31032633
- Wang X et al. Fatty acid receptor GPR120 promotes breast cancer chemoresistance by upregulating ABC transporters expression and fatty acid synthesis. EBioMedicine 40:251-262 (2019). PubMed: 30738829