Anti-Mre11 (phospho S678)抗体(ab77631)

概述

  • 产品名称Anti-Mre11 (phospho S678)抗体
    参阅全部 Mre11 一抗
  • 描述
    兔多克隆抗体to Mre11 (phospho S678)
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Non Human Primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Mre11, phosphorylated at S678.

    (Peptide available as ab112241.)

  • 阳性对照
    • This antibody gave a positive signal in Jurkat Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab77631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 81 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
    • 疾病相关Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
    • 序列相似性Belongs to the MRE11/RAD32 family.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AT like disease antibody
      • Ataxia telangiectasia disorder like antibody
      • ATLD antibody
      • DNA recombination and repair protein antibody
      • Double strand break repair protein MRE11A antibody
      • Double-strand break repair protein MRE11A antibody
      • endo/exonuclease Mre11 antibody
      • HNGS1 antibody
      • meiotic recombination (S. cerevisiae) 11 homolog A antibody
      • Meiotic recombination 11 homolog 1 antibody
      • meiotic recombination 11 homolog A (S. cerevisiae) antibody
      • Meiotic recombination 11 homolog A antibody
      • MmMRE11A antibody
      • Mre 11 antibody
      • MRE 11a antibody
      • MRE 11b antibody
      • MRE11 homolog 1 antibody
      • MRE11 homolog A antibody
      • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
      • MRE11 meiotic recombination 11 homolog A antibody
      • MRE11_HUMAN antibody
      • MRE11A antibody
      • MRE11b antibody
      • OTTHUMP00000236830 antibody
      • OTTHUMP00000236831 antibody
      • OTTHUMP00000236832 antibody
      • OTTHUMP00000236833 antibody
      see all

    Anti-Mre11 (phospho S678) antibody 图像

    • All lanes : Anti-Mre11 (phospho S678) antibody (ab77631) at 1 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human Mre11 (phospho S678) peptide (ab112241) at 1 µg/ml
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human Mre11 peptide (ab116269) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 81 kDa
      Observed band size : 85 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 140 kDa,50 kDa,70 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 20 minutes

    Anti-Mre11 (phospho S678) antibody (ab77631)参考文献

    ab77631 has not yet been referenced specifically in any publications.

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