The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
功能Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
疾病相关Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
序列相似性Belongs to the MRE11/RAD32 family.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
Double strand break repair protein MRE11A antibody
Double-strand break repair protein MRE11A antibody
endo/exonuclease Mre11 antibody
meiotic recombination (S. cerevisiae) 11 homolog A antibody
Meiotic recombination 11 homolog 1 antibody
meiotic recombination 11 homolog A (S. cerevisiae) antibody
Meiotic recombination 11 homolog A antibody
Mre 11 antibody
MRE 11a antibody
MRE 11b antibody
MRE11 homolog 1 antibody
MRE11 homolog A antibody
MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
MRE11 meiotic recombination 11 homolog A antibody
Anti-Mre11 antibody - ChIP Grade 图像
ChIP - Mre11 antibody - ChIP Grade (ab12159)
A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin.
Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR Green (Invitrogen), and detected using a Fuji scanning fluorometer.
Anti-Mre11 antibody - ChIP Grade (ab12159)参考文献
has not yet been referenced specifically in any publications.