概述

  • 产品名称Anti-Mre11抗体
    参阅全部 Mre11 一抗
  • 描述
    兔多克隆抗体to Mre11
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Ferret, Gorilla, Opossum, Bat
  • 免疫原

    Synthetic peptide (Human) - which represents a portion of Meiotic Recombination 11 (Mre 11)encoded within exon 3.

  • 阳性对照
    • HeLa cell nuclear extract.

性能

应用

Our Abpromise guarantee covers the use of ab3621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • 疾病相关Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
  • 序列相似性Belongs to the MRE11/RAD32 family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AT like disease antibody
    • Ataxia telangiectasia disorder like antibody
    • ATLD antibody
    • DNA recombination and repair protein antibody
    • Double strand break repair protein MRE11A antibody
    • Double-strand break repair protein MRE11A antibody
    • endo/exonuclease Mre11 antibody
    • HNGS1 antibody
    • meiotic recombination (S. cerevisiae) 11 homolog A antibody
    • Meiotic recombination 11 homolog 1 antibody
    • meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • Meiotic recombination 11 homolog A antibody
    • MmMRE11A antibody
    • Mre 11 antibody
    • MRE 11a antibody
    • MRE 11b antibody
    • MRE11 homolog 1 antibody
    • MRE11 homolog A antibody
    • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • MRE11 meiotic recombination 11 homolog A antibody
    • MRE11_HUMAN antibody
    • MRE11A antibody
    • MRE11b antibody
    • OTTHUMP00000236830 antibody
    • OTTHUMP00000236831 antibody
    • OTTHUMP00000236832 antibody
    • OTTHUMP00000236833 antibody
    see all

Anti-Mre11 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Mre11 with ab3621 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 81 kDa

    Sample: Nuclear extract (25µg) from Hela cells

    Affinity purified Rabbit anti-Mre11 (ab3621) used at 1:4,000 dilution

     

     

    Sample: Nuclear extract (25µg) from Hela cells

    Affinity purified Rabbit anti-Mre11 (ab3621) used at 1:4,000 dilution

Anti-Mre11 antibody (ab3621)参考文献

This product has been referenced in:
  • Hematulin A  et al. NBS1 is required for IGF-1 induced cellular proliferation through the Ras/Raf/MEK/ERK cascade. Cell Signal 20:2276-2285 (2008). Read more (PubMed: 18793719) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry regarding ab3621.

I can confirm that ab3621 was produced using a peptide identical for human and bovine. Since its cross-reactivity has not been tested with bovine (only on human samples) this is an untested species...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa and Indian muntjac)
Specification HeLa and Indian muntjac
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Apr 13 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"