Anti-Mre11抗体(ab30725)

概述

  • 产品名称Anti-Mre11抗体
    参阅全部 Mre11 一抗
  • 描述
    兔多克隆抗体to Mre11
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Mre11.

    (Peptide available as ab33123.)

  • 阳性对照
    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate and A431 (Human epithelial carcinoma cell line) Whole Cell Lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab30725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
IHC-P
  • 应用说明IHC-P: Use at a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    ICC/IF: Use at a concentration of 1 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
    • 疾病相关Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
    • 序列相似性Belongs to the MRE11/RAD32 family.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AT like disease antibody
      • Ataxia telangiectasia disorder like antibody
      • ATLD antibody
      • DNA recombination and repair protein antibody
      • Double strand break repair protein MRE11A antibody
      • Double-strand break repair protein MRE11A antibody
      • endo/exonuclease Mre11 antibody
      • HNGS1 antibody
      • meiotic recombination (S. cerevisiae) 11 homolog A antibody
      • Meiotic recombination 11 homolog 1 antibody
      • meiotic recombination 11 homolog A (S. cerevisiae) antibody
      • Meiotic recombination 11 homolog A antibody
      • MmMRE11A antibody
      • Mre 11 antibody
      • MRE 11a antibody
      • MRE 11b antibody
      • MRE11 homolog 1 antibody
      • MRE11 homolog A antibody
      • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
      • MRE11 meiotic recombination 11 homolog A antibody
      • MRE11_HUMAN antibody
      • MRE11A antibody
      • MRE11b antibody
      • OTTHUMP00000236830 antibody
      • OTTHUMP00000236831 antibody
      • OTTHUMP00000236832 antibody
      • OTTHUMP00000236833 antibody
      see all

    Anti-Mre11 antibody 图像

    • All lanes : Anti-Mre11 antibody (ab30725) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat whole cell lysate (ab7899)
      Lane 3 : A431 whole cell lysate (ab7909)

      Lysates/proteins at 20 µg per lane.

      Secondary
      IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size : 81 kDa
      Observed band size : 81 kDa
    • ICC/IF image of ab30725 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab30725, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    • IHC image of Mre11 staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30725, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    Anti-Mre11 antibody (ab30725)参考文献

    ab30725 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (LCLS)
    Loading amount 50 µg
    Specification LCLS
    Gel Running Conditions Reduced Denaturing (6% gel)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mr. Ed miller

    Verified customer

    提交于 Apr 02 2009

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    提交于 Dec 18 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"