概述

  • 产品名称Anti-Mre11抗体[12D7]
    参阅全部 Mre11 一抗
  • 描述
    小鼠单克隆抗体[12D7] to Mre11
  • 经测试应用适用于: Flow Cyt, ICC/IF, IHC-Fr, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Amino acids 182-582 of Mre11 expressed in E. coli.

  • 阳性对照
    • T24 cells, recombinant fusion protein.

性能

应用

Our Abpromise guarantee covers the use of ab214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1-2µg for 106 cells.
ICC/IF 1/200 - 1/500. Wash cells with PBS, then wash with PBS containing 0.5% Triton X-100 and fix for 5 min with PBS containing 3% paraformaldehyde. Block cells for 1 h in PBS containing 15% fetal bovine serum (see Robinson et al).
IHC-Fr 1/200.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). (see Robinson et al).
IP Use a concentration of 1 - 5 µg/ml. (for normal lymphoblastoid cell lines).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • 疾病相关Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
  • 序列相似性Belongs to the MRE11/RAD32 family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AT like disease antibody
    • Ataxia telangiectasia disorder like antibody
    • ATLD antibody
    • DNA recombination and repair protein antibody
    • Double strand break repair protein MRE11A antibody
    • Double-strand break repair protein MRE11A antibody
    • endo/exonuclease Mre11 antibody
    • HNGS1 antibody
    • meiotic recombination (S. cerevisiae) 11 homolog A antibody
    • Meiotic recombination 11 homolog 1 antibody
    • meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • Meiotic recombination 11 homolog A antibody
    • MmMRE11A antibody
    • Mre 11 antibody
    • MRE 11a antibody
    • MRE 11b antibody
    • MRE11 homolog 1 antibody
    • MRE11 homolog A antibody
    • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • MRE11 meiotic recombination 11 homolog A antibody
    • MRE11_HUMAN antibody
    • MRE11A antibody
    • MRE11b antibody
    • OTTHUMP00000236830 antibody
    • OTTHUMP00000236831 antibody
    • OTTHUMP00000236832 antibody
    • OTTHUMP00000236833 antibody
    see all

Anti-Mre11 antibody [12D7] 图像

  • ab214 staining Mre11 in human normal and gastric cancer tissue sections (20µm) by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde for 3 hours, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/200 in 3% BSA + 0.1% Triton X-100 in TBS buffer) for 12 hours at 4°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

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  • All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/500 dilution

    Lane 1 : Mouse breast cancer cell line - whole cell lysate. Transfected with vector control.
    Lane 2 : Mouse breast cancer cell line - whole cell lysate. Transfected with knockdown shRNA targeting Mre11 gene.

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated Goat anti-mouse IgG polyclonal at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 79 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells.
    (Panel D - negative control).



  • Predicted band size : 79 kDa

    This picture was kindly supplied as part of the reviews for ab214 and for ab89 submitted by Anya Polischouk.

    The bands represent nuclear (N) and cytoplasmic (C) extract from human lung cancer cells (U1810).

     

  • ab214 at 1/200 staining normal human bronchus tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in EDTA was peformed. The tissue was blocked in normal rabbit serum and then incubated with the antibody for 1 hour. An HRP conjugated goat polyclonal antibody was used as the secondary.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab214, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Mre11 antibody [12D7] (ab214)参考文献

This product has been referenced in:
  • Chen WT  et al. ATM regulation of IL-8 links oxidative stress to cancer cell migration and invasion. Elife 4:N/A (2015). WB ; Human . Read more (PubMed: 26030852) »
  • Becker A  et al. ATM alters the otherwise robust chromatin mobility at sites of DNA double-strand breaks (DSBs) in human cells. PLoS One 9:e92640 (2014). WB, IF ; Human . Read more (PubMed: 24651490) »

See all 28 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (LLC-PK1 cell line)
Permeabilization Yes - 0.5% Triton-X 100
Specification LLC-PK1 cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: R.T°C
Fixative Paraformaldehyde
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提交于 Feb 10 2016

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Human Tissue sections (Normal or gastric cancer tissues)
Specification Normal or gastric cancer tissues
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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提交于 Feb 20 2014

Application Immunocytochemistry
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Sample Mouse Cultured Cells (Mouse embryonic fibroblasts)
Specification Mouse embryonic fibroblasts
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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提交于 Feb 19 2014

Application Immunocytochemistry
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Human Cultured Cells (Hela cell line)
Specification Hela cell line
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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提交于 Feb 18 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Sample Human Cell (Human colon cancer cell lines)
Specification Human colon cancer cell lines
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
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提交于 Oct 15 2013

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (7.5)
Sample Mouse Cell lysate - whole cell (Mouse breast cancer cell line)
Specification Mouse breast cancer cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Oct 15 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 15 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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提交于 Nov 08 2011

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Application Western blot
Sample Human Cell lysate - nuclear (human cancer cells (colon, bladder))
Loading amount 20 µg
Specification human cancer cells (colon, bladder)
Gel Running Conditions Reduced Denaturing (4-12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
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提交于 Apr 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 20 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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提交于 Nov 17 2010

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Normal Bronchus)
Specification Normal Bronchus
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5%
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提交于 Jun 15 2007

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