概述

  • 产品名称Anti-MRas抗体
    参阅全部 MRas 一抗
  • 描述
    兔多克隆抗体to MRas
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide:

    KKKKTKWRGDRATGTHKLQ

    , corresponding to C terminal amino acids 186-204 of Human MRas

  • 阳性对照
    • HeLa or HEK293 cells.

性能

应用

Our Abpromise guarantee covers the use of ab26303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 24 kDa). Block with 5% milk and dilute in TBST only.
ICC/IF 1/1000.

靶标

  • 功能May serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway.
  • 组织特异性Expression highly restricted to the brain and heart.
  • 序列相似性Belongs to the small GTPase superfamily. Ras family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ42964 antibody
    • M ras antibody
    • M-ras antibody
    • Mras antibody
    • Muscle and microspikes Ras antibody
    • Muscle RAS oncogene homolog antibody
    • Muscle Ras oncogene homologue antibody
    • Muscle Ras viral oncogene homolog antibody
    • R ras3 antibody
    • R-ras3 antibody
    • Ras related protein MRas antibody
    • Ras related protein RRas3 antibody
    • Ras-related protein M-Ras antibody
    • Ras-related protein R-Ras3 antibody
    • RASM_HUMAN antibody
    • Related Ras viral oncogene homolog 3 antibody
    • RRas3 antibody
    • XRas antibody
    see all

Anti-MRas antibody 图像

  • All lanes : Anti-MRas antibody (ab26303)

    Lane 1 : HEK 293T cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : HeLa cell lysate

    Secondary
    anti-rabbit HRP conjugated
    Developed using the ECL technique

    Predicted band size : 24 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab26303 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26303, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab26303 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab26303, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-MRas antibody (ab26303)参考文献

ab26303 has not yet been referenced specifically in any publications.

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