概述

  • 产品名称Anti-MRAP抗体
    参阅全部 MRAP 一抗
  • 描述
    兔多克隆抗体to MRAP
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human MRAP aa 7-36 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • 293 cell lysate. Human breast carcinoma. This antibody gave a positive result when used in the following methanol fixed cell lines: U-87 MG

性能

应用

Our Abpromise guarantee covers the use of ab103319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 19 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Required for MC2R expression in certain cell types, suggesting that it is involved in the processing, trafficking or function of MC2R. May be involved in the intracellular trafficking pathways in adipocyte cells.
  • 组织特异性Expressed in adrenal cortex, testis, breast, thyroid, lymph node, ovary and fat. Expressed in adipose tissues.
  • 疾病相关Defects in MRAP are the cause of glucocorticoid deficiency type 2 (GCCD2) [MIM:607398]; also known as familial glucocorticoid deficiency type 2 (FGD2). GCCD2 is an autosomal recessive disorder due to congenital insensitivity or resistance to adrenocorticotropin (ACTH). It is characterized by progressive primary adrenal insufficiency, without mineralocorticoid deficiency.
  • 细胞定位Cytoplasm > perinuclear region. Cytoplasm. Cell membrane. Endoplasmic reticulum. Concentrated at the perinuclear membrane region. Upon insulin stimulation, it is redistributed into spotty structures throughout the cytoplasm (By similarity). Localizes both to plasma membrane and endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B27 antibody
    • C21orf61 antibody
    • FALP antibody
    • Fat cell-specific low molecular weight protein antibody
    • Fat tissue-specific low MW protein antibody
    • FGD2 antibody
    • GCCD2 antibody
    • Melanocortin-2 receptor accessory protein antibody
    • Mrap antibody
    • MRAP_HUMAN antibody
    see all

Anti-MRAP antibody 图像

  • ab103319 stained U-87 MG cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103319 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-MRAP antibody (ab103319) at 1/100 dilution + 293 cell lysate at 35 µg

    Predicted band size : 19 kDa
  • ab103319, at 1/50 dilution, staining MRAP in Human breast carcinoma by immunohistochemistry.

Anti-MRAP antibody (ab103319)参考文献

ab103319 has not yet been referenced specifically in any publications.

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