概述

  • 产品名称Anti-Mps1抗体
    参阅全部 Mps1 一抗
  • 描述
    兔多克隆抗体to Mps1
  • 特异性ab24227 reacts with TTK (MPS1) protein. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide:

    LVGLNSPNSILKAAKTLYEHYSGGESHNSSSSKTFEKKRGKK

    , corresponding to C terminal amino acids 800-841 of Human Mps1

  • 阳性对照
    • Hela cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab24227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2500 - 1/25000. Predicted molecular weight: 95 kDa.
IP Use at an assay dependent dilution.

靶标

  • 功能Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
  • 组织特异性Present in rapidly proliferating cell lines.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cancer/testis antigen 96 antibody
    • CT96 antibody
    • Dual specificity protein kinase TTK antibody
    • ECSTY kinase antibody
    • ESK antibody
    • FLJ38280 antibody
    • hMPS1 antibody
    • Monopolar Spindle 1 Like 1 antibody
    • MPH1 antibody
    • Mps 1 antibody
    • MPS1L1 antibody
    • Phosphotyrosine Picked Threonine Kinase antibody
    • Phosphotyrosine picked threonine-protein kinase antibody
    • PYT antibody
    • RP3-357D13.2 antibody
    • ttk antibody
    • TTK Protein Kinase antibody
    • TTK_HUMAN antibody
    see all

Anti-Mps1 antibody 图像

  • All lanes : Anti-Mps1 antibody (ab24227)

    Lane 1 : HeLa cell lyste at 15µg and ab at 0.033ug/ml
    Lane 2 : HeLa cell lyste at 50ug and ab at 0.033ug/ml
    Lane 3 : HeLa cell lyste at 50ug and ab at 0.33ug/ml
    Lane 4 : HeLa cell lyste at 50ug and ab at 0.33ug/ml
    Lane 5 : HeLa cell lyste at 2mg and ab at 1ug/2mg lysate
    Lane 6 : HeLa cell lyste at 2mg and ab at 1ug/2mg lysate
    Lane 7 : HeLa cell lyste at 2mg and ab at 1ug/2mg lysate

    Developed using the ECL technique

    Predicted band size : 95 kDa
    Observed band size : 95 kDa
  • ICC/IF image of ab24227 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24227, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Mps1 antibody (ab24227)参考文献

ab24227 has not yet been referenced specifically in any publications.

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