The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IP: 1-4 µg/mg lysate.
WB: 1/2500 - 1/25000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
组织特异性Present in rapidly proliferating cell lines.
序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.