The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
相关性MMPED2 (Metallophosphoesterase domain containing 2) displays low metallophosphoesterase activity (in vitro). It is predominantly expressed in the fetal brain and is thought to have a role in development of the nervous system. Alternatively spliced transcript variants have been described.
Metallophosphoesterase domain containing protein 2 antibody
MPPED 2 antibody
Anti-MPPED2 antibody 图像
Western blot - MPPED2 antibody (ab87077)
Anti-MPPED2 antibody (ab87077) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 33 kDa Observed band size : 33 kDa
Anti-MPPED2 antibody (ab87077)参考文献
This product has been referenced in:
Shen L et al. miR-448 downregulates MPPED2 to promote cancer proliferation and inhibit apoptosis in oral squamous cell carcinoma. Exp Ther Med12:2747-2752 (2016).
Read more (PubMed: 27698780) »