概述

  • 产品名称Anti-MPP5抗体
    参阅全部 MPP5 一抗
  • 描述
    山羊多克隆抗体to MPP5
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    NKASPPFPLISNAQ

    , corresponding to N terminal amino acids 174-187 of Human MPP5.

  • 阳性对照
    • Human Brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab20511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
ELISA 1/32000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 77 kDa).

靶标

  • 功能May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton.
  • 组织特异性Expressed in the retina (at protein level).
  • 序列相似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 2 L27 domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SH3 domain.
  • 结构域The L27 domain 1 functions in targeting to the tight junctions by binding to INADL.
    The PDZ domain binds to the C-terminus of SC6A1.
  • 细胞定位Cell membrane. Endomembrane system. Cell junction > tight junction. Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membrane (OLM), apical to the adherens junction (AJ), where it colocalizes with CRB1. Colocalizes with MPP1 in the retina at the outer limiting membrane (OLM). Localized to the Purkinje cell body and axon.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ12615 antibody
    • MAGUK p55 subfamily member 5 antibody
    • Membrane protein palmitoylated 5 antibody
    • MPP 5 antibody
    • MPP5 antibody
    • MPP5_HUMAN antibody
    • PALS 1 antibody
    • PALS1 antibody
    • Protein associated with Lin-7 antibody
    • Stardust (Drosophila) antibody
    • Stardust antibody
    see all

Anti-MPP5 antibody 图像

  • Anti-MPP5 antibody (ab20511) at 1 µg/ml + Human brain lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 77 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ab20511 at 5ug/ml staining MPP5 in human skeletal tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

Anti-MPP5 antibody (ab20511)参考文献

ab20511 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic dorsal forebrain)
Permeabilization Yes - Triton 0.5%
Specification Embryonic dorsal forebrain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
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提交于 Aug 10 2016

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