概述

  • 产品名称Anti-MPP5抗体
    参阅全部 MPP5 一抗
  • 描述
    兔多克隆抗体to MPP5
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Horse, Chicken, Cow, Dog, Human, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Mouse MPP5.

  • 阳性对照
    • This antibody gave a positive signal in the following Mouse tissue lysates: Hippocampus; Cerebellum; Lung; Brain; Sciatic Nerve.

性能

应用

Our Abpromise guarantee covers the use of ab106850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 77 kDa). Abcam recommends using milk as the blocking agent (3%).

靶标

  • 功能May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton.
  • 组织特异性Expressed in the retina (at protein level).
  • 序列相似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 2 L27 domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SH3 domain.
  • 结构域The L27 domain 1 functions in targeting to the tight junctions by binding to INADL.
    The PDZ domain binds to the C-terminus of SC6A1.
  • 细胞定位Cell membrane. Endomembrane system. Cell junction > tight junction. Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membrane (OLM), apical to the adherens junction (AJ), where it colocalizes with CRB1. Colocalizes with MPP1 in the retina at the outer limiting membrane (OLM). Localized to the Purkinje cell body and axon.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ12615 antibody
    • MAGUK p55 subfamily member 5 antibody
    • Membrane protein palmitoylated 5 antibody
    • MPP 5 antibody
    • MPP5 antibody
    • MPP5_HUMAN antibody
    • PALS 1 antibody
    • PALS1 antibody
    • Protein associated with Lin-7 antibody
    • Stardust (Drosophila) antibody
    • Stardust antibody
    see all

Anti-MPP5 antibody 图像

  • All lanes : Anti-MPP5 antibody (ab106850) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : Mouse Hippocampus Tissue Lysate
    Lane 2 : Cerebellum Mouse Tissue Lysate
    Lane 3 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 4 : Brain (Mouse) Tissue Lysate
    Lane 5 : Mouse Sciatic Nerve Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 77 kDa
    Observed band size : 83 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-MPP5 antibody (ab106850)参考文献

ab106850 has not yet been referenced specifically in any publications.

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