概述

  • 产品名称Anti-MPG抗体
    参阅全部 MPG 一抗
  • 描述
    小鼠单克隆抗体to MPG
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: MPARSGAQFC RRMGQKKQRP ARAGQPHSSS DAAQAPAEQP HSSSDAAQAP CPRERCLGPP TTPGPYRSIY FSSPKGHLTR LGLEFFDQPA , corresponding to amino acids 1-90 of Human MPG

性能

应用

Our Abpromise guarantee covers the use of ab55461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 33 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
  • 序列相似性Belongs to the DNA glycosylase MPG family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3 alkyladenine DNA glycosylase antibody
    • 3-alkyladenine DNA glycosylase antibody
    • 3-methyladenine DNA glycosidase antibody
    • 3MG_HUMAN antibody
    • AAG antibody
    • ADPG antibody
    • Alkyladenine DNA glycosylase antibody
    • anpg antibody
    • APNG antibody
    • CRA36.1 antibody
    • DNA 3 methyladenine glycosylase antibody
    • DNA-3-methyladenine glycosylase antibody
    • MDG antibody
    • Mid1 antibody
    • Mpg antibody
    • N methylpurine DNA glycosirase antibody
    • N methylpurine DNA glycosylase antibody
    • N-methylpurine-DNA glycosylase antibody
    • PIG11 antibody
    • PIG16 antibody
    • Proliferation inducing protein 11 antibody
    • Proliferation inducing protein 16 antibody
    see all

Anti-MPG antibody 图像



  • Predicted band size : 33 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MPG knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab55461 observed at 35 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab55461 was shown to specifically react with MPG when MPG knockout samples were used. Wild-type and MPG knockout samples were subjected to SDS-PAGE. ab55461 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • MPG antibody (ab55461) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.


  • Predicted band size : 33 kDa
    MPG antibody (ab55461) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55461 staining MPG in human mammary tissue by Immunohistochemistry (paraffin embedded sections).
    Paraffin-embedded blocks were sectioned and mounted on frost-free slides. The 3-10 µm sections were deparaffinized in xylene and rehydrated through a series of graded alcohols. Slides were washed with 1× PBS and endogenous peroxidases were blocked with 1.5% hydrogen peroxide in 1× PBS for 20 minutes at 25°C. After three 5 minutes washes in 1× PBS, slides were incubated in blocking solution (1× PBS with 0.1% Triton X-100, 3% bovine serum albumin) with 5% normal donkey serum for 10 minutes at 25°C. Control (no primary antibody) and experimental slides were incubated overnight at 4°C, respectively, in blocking solution alone or blocking solution with ab55461 at 1/600 dilution. Biotin-conjugated secondary antibody 1/200 was added and slides were incubated at 25°C for 30 minutes and then washed three times with 1× PBS. The ABC Peroxidase Staining kit (1:100 dilution of each Reagent A

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa


    Exposure time : 10 seconds
  • Overlay histogram showing HeLa cells stained with ab55461 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55461, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemical analysis of Human gliomas, staining MPG with ab55461.

    Formalin fixed, paraffin-embedded tissue was blocked with 5% normal horse serum and incubated with primary antibody (1/500) overnight at 4°C. Staining was detected using DAB.

Anti-MPG antibody (ab55461)参考文献

This product has been referenced in:
  • Sousa MM  et al. An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells. PLoS One 8:e55493 (2013). WB ; Human . Read more (PubMed: 23405159) »
  • Liu C  et al. Aberrant expression of N-methylpurine-DNA glycosylase influences patient survival in malignant gliomas. J Biomed Biotechnol 2012:760679 (2012). WB, IHC-P ; Human . Read more (PubMed: 22496614) »

See all 4 Publications for this product

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Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

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Thank you very much for your reply. My colleague is out of the office today but I'll be happy to help you with this.

We don't currently have blocking peptides available for ab105340 or ab56540, but I'm in the process of adding the peptide for...

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Thank you for your reply. The publications that are known to reference an Abcam product can be found on our online datasheets on the specific references tab. Below are PubMed IDs for the antibodies you are interested in.

MGMT (ab39253) - 2175...

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Thank you for contacting us.

The final dilutions should be optimized for your particular samples and protocols, but the following would be my recommended starting dilutions for WB with an overnight incubation:

MGMT (ab39253) - 1:500...

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