概述

  • 产品名称Anti-MOX1抗体
    参阅全部 MOX1 一抗
  • 描述
    兔多克隆抗体to MOX1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH, from the N terminal region of MOX1.

  • 阳性对照
    • 293 cell line lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab75895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 28 kDa.Can be blocked with MOX1 peptide (ab199249).
ICC/IF 1/10 - 1/50.

靶标

  • 功能Mesodermal transcription factor that plays a key role in somitogenesis and is specifically required for sclerotome development. Required for maintenance of the sclerotome polarity and formation of the cranio-cervical joints (PubMed:23290072, PubMed:24073994). Binds specifically to the promoter of target genes and regulates their expression. Activates expression of NKX3-2 in the sclerotome. Activates expression of CDKN1A and CDKN2A in endothelial cells, acting as a regulator of vascular cell proliferation. While it activates CDKN1A in a DNA-dependent manner, it activates CDKN2A in a DNA-independent manner. Required for hematopoietic stem cell (HSCs) induction via its role in somitogenesis: specification of HSCs occurs via the deployment of a specific endothelial precursor population, which arises within a sub-compartment of the somite named endotome.
  • 疾病相关Klippel-Feil syndrome 2, autosomal recessive
  • 序列相似性Contains 1 homeobox DNA-binding domain.
  • 细胞定位Nucleus. Cytoplasm. Localizes predominantly in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homeobox protein MOX-1 antibody
    • MEOX1 antibody
    • MEOX1_HUMAN antibody
    • Mesenchyme homeo box 1 antibody
    • Mesenchyme homeobox 1 antibody
    • MOX 1 antibody
    • MOX1 antibody
    see all

Anti-MOX1 antibody 图像

  • Immunofluorescence of HeLa cells labelling MOX1 with ab75895. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab75895 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃) and cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MOX1 immunoreactivity is localised to the nucleolus.

  • Anti-MOX1 antibody (ab75895) at 1/230 dilution + 293 cell line lysate at 35 µg

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

Anti-MOX1 antibody (ab75895)参考文献

This product has been referenced in:
  • Thiaville MM  et al. Identification of PBX1 target genes in cancer cells by global mapping of PBX1 binding sites. PLoS One 7:e36054 (2012). Read more (PubMed: 22567123) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab75895.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"