The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).Can be blocked with MOX1 peptide (ab199250). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Mesodermal transcription factor that plays a key role in somitogenesis and is specifically required for sclerotome development. Required for maintenance of the sclerotome polarity and formation of the cranio-cervical joints (PubMed:23290072, PubMed:24073994). Binds specifically to the promoter of target genes and regulates their expression. Activates expression of NKX3-2 in the sclerotome. Activates expression of CDKN1A and CDKN2A in endothelial cells, acting as a regulator of vascular cell proliferation. While it activates CDKN1A in a DNA-dependent manner, it activates CDKN2A in a DNA-independent manner. Required for hematopoietic stem cell (HSCs) induction via its role in somitogenesis: specification of HSCs occurs via the deployment of a specific endothelial precursor population, which arises within a sub-compartment of the somite named endotome.
Klippel-Feil syndrome 2, autosomal recessive
Contains 1 homeobox DNA-binding domain.
Nucleus. Cytoplasm. Localizes predominantly in the nucleus.
ab23279, at 4.0 - 8.0ug/ml dilution, staining human MOX1 in human stomach by Immunohistochemistry, Paraffin embedded tissue. Cells with positive staining, marked by arrows, are Epithelial cells of Fundic Gland. Magnification 400 x.